|Product Name||Rat GRP78 ELISA Kit|
|Description||Colorimetric detection of GRP78 in rat lysates. 96wells/kit, with removable strips.|
|Cite This Product||Rat GRP78 ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7117)|
|Cross Reactivity||There is no detectable cross-reactivity.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
|Assay Range||0 - 50 ng/ml|
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Storage||Store at 4°C.|
|Anti-Rat GRP78 Immunoassay Plate||1 Plate|
|Recombinant Rat GRP78 Standard||2 vials/ 56.7ng|
|Standard and Sample Diluent||1 vial/ 50 ml|
|Anti-Rat Grp78 Biotinylated Antibody Concentrate||1 vial/150?L|
|10X Wash Buffer Concentrate||1 vial/100 ml|
|Anti-Rat GRP78 Biotinylated Antibody Diluent||1 vial/ 13 ml|
|Streptavidin Poly HRP Concentrate||1 vial/150 μl|
|Streptavidin Poly HRP Diluent||1 vial/ 13 ml|
|TMB Substrate||1 vial/ 13 ml|
|Stop Solution||1 vial/ 13 ml|
|Protein Name||Endoplasmic reticulum chaperone BiP|
|Protein Function||Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (By similarity). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).|
|Subcellular Localization||Endoplasmic reticulum lumen.|
|Alternative Names||Endoplasmic reticulum chaperone BiP|
|Research Areas||Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Organelle Markers, Protein Trafficking||
GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5’ nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival. In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer’s patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti?tumor drugs.
Typical Standard Curve for the Rat GRP78 ELISA Kit (Enzyme-Linked Immunosorbent Assay)–EK7117 Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0 – 50 ng/ml.