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Rat CD36/SR-B3 ELISA Kit

CD36 antigen|CD36 antigen (collagen type I receptor thrombospondin receptor)|CD36 molecule|CD36 molecule (thrombospondin receptor)|CHDS7|Cluster determinant 36|FAT|Fatty acid translocase|Glycoprotein


ELISA试剂盒 - Rat Elisa Kit











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Product Brief

  • 产品概况

    产品名称 Rat CD36/SR-B3 ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD36/SR-B3
    引用此产品 Rat CD36/SR-B3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1299)
    验证种属 Rat
    免疫原 标准品表达系统:CHO,G30-K439
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit


    敏感性 <10pg/ml
    检测范围 156pg/ml-10000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from mouse
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了CD36的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)


    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- rat CD36 antibody1
    ST0000-10lyophilized recombinant rat CD36 standard10ng/tubex2
    AR1107biotinylated anti- rat CD36 antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4


    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 CD36
    蛋白名 Platelet glycoprotein 4
    基因全名 Platelet glycoprotein 4
    蛋白功能 Binds to collagen, thrombospondin, anionic phospholipidsand oxidized low-density lipoprotein (oxLDL). May function as acell adhesion molecule. Directly mediates cytoadherence ofPlasmodium falciparum parasitized erythrocytes. Binds long chainfatty acids and may function in the transport and/or as aregulator of fatty acid transport (By similarity). Receptor forthombospondins, THBS1 AND THBS2, mediating their antiangiogeniceffects (By similarity). As a coreceptor for TLR4-TLR6, promotesinflammation in monocytes/macrophages. Upon ligand binding, suchas oxLDL or amyloid-beta 42 binding, rapidly induces the formationof a heterodimer of TLR4 and TLR6, which is internalized andtriggers inflammatory signals, leading to the NF-kappa-B-dependentproduction of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signalingpathway, and CCL5 cytokine, via TICAM1 signaling pathway, as wellas IL1B secretion..
    Tissue Specificity Expressed at high levels in heart, intestine,spleen, adipose tissue, skeletal muscle and, at lower levels, intestes..
    Subcellular Localization Cell membrane; Upon ligand-binding, internalized through dynamin-dependentendocytosis..
    Uniprot ID CD36: Q07969
    指标别名 Platelet glycoprotein 4; Adipocyte membrane protein; Fatty acid translocase; Fatty acid transport protein; Glycoprotein IIIb; GPIIIB; PAS IV; PAS-4; Platelet glycoprotein IV; GPIV; CD36; Cd36; Fat;
    相关研究领域 cardiovascular|blood|platelets|immunology|cell type markers|cd|microbiology|protein|human protein|defensin|stem cells|hematopoietic progenitors|hematopoietic stem cells|human lineage negative|atherosclerosis|lipid transport|endothelial progenitors|endothelial markers|cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|types of disease|heart disease

    Platelet glycoprotein 4指标简介

    CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.


    Intra-Assay PrecisionInter-Assay Precision
    Standard deviation8.0136.50136.0911.8962.45239.45

    Rat CD36/SR-B3 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30min)






0.01M TBS洗涤3次。


0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。


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