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Rat BMP-7 ELISA Kit

BMP 7|BMP7|Bone morphogenetic protein 7|Eptotermin alfa|OP 1|OP1|Osteogenic protein 1|OTTHUMP00000166094

分类:

ELISA试剂盒 - Rat Elisa Kit

说明书:

EK1444

2000元/96T  

CCS/S/P/BT

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称Rat BMP-7 ELISA Kit
    产品描述Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat BMP-7
    引用此产品Rat BMP-7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1444)
    验证种属Rat
    免疫原标准品表达系统:CHO,S292-H430
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    规格96 wells/kit

    产品性质

    敏感性<10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围31.2pg/ml-2000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型bone tissue, cell culture supernates, serum and plasma(heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    一般表达浓度以下文章提供了Bmp7的表达量的信息

    保存条件4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU名称数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- rat BMP-7 antibody1
    ST0000-10Lyophilized recombinant rat BMP-7 standard10ng/tube x 2
    AR1107Biotinylated anti- rat BMP-7 antibody130&mu;l(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130&mu;l(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4



    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    1. 96孔读板仪器。

    2. 自动洗板机.

    3. 移液器和枪头。建议使用多通道移液器/排枪。

    4. Eppendorf 管。

    5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

    0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

    基因名Bmp7
    蛋白名Bone morphogenetic protein 7
    基因全名bone morphogenetic protein 7
    Subcellular LocalizationSecreted.
    Uniprot IDBmp7: G3V6W8
    指标别名Bone morphogenetic protein 7; Protein Bmp7; Bmp7; rCG_40866;
    相关研究领域signal transduction|growth factors/hormones|tgf|cytoskeleton / ecm|extracellular matrix|structures|bone|cell biology|cell cycle|cell differentiation|stem cells|signaling pathways|tgf beta|secreted|cancer|developmental biology|organogenesis|nervous system development|excretory system development|kidney development|metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism

    bone morphogenetic protein 7指标简介

    Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-?? superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

    批内差/批间差


    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)13560912881987161563
    Standard deviation6.0832.2866.9815.0558.71109.41
    CV(%)4.55.35.27.68.27.0

    Rat BMP-7 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25min)

    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.0840.1290.1710.2330.3910.7121.3202.240

    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。


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