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Rat ALCAM ELISA Kit

ALCAM protein | CD166 antigen | Protein MEMD | Q13740

分类:

ELISA试剂盒 - Rat Elisa Kit

说明书:

EK1572

2000元/96T

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Rat ALCAM ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat ALCAM
    引用此产品 Rat ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1572)
    验证种属 Rat
    免疫原 标准品表达系统:NSO,W28-K527
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 78pg/ml-5000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    一般表达浓度 以下文章提供了Alcam的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- rat ALCAM antibody1
    ST0000-10Lyophilized recombinant rat ALCAM standard10ng/tube??2
    AR1107Biotinylated anti- rat ALCAM antibody130??l(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130??l(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 Alcam
    蛋白名 CD166 antigen
    基因全名 CD166 antigen
    蛋白功能 Cell adhesion molecule that mediates both heterotypiccell-cell contacts via its interaction with CD6, as well ashomotypic cell-cell contacts. Promotes T-cell activation andproliferation via its interactions with CD6 (By similarity).Contributes to the formation and maturation of the immunologicalsynapse via its interactions with CD6 (By similarity). Mediateshomotypic interactions with cells that express ALCAM. Mediatesattachment of dendritic cells onto endothelial cells via homotypicinteraction. Inhibits endothelial cell migration and promotesendothelial tube formation via homotypic interactions. Requiredfor normal organization of the lymph vessel network. Required fornormal hematopoietic stem cell engraftment in the bone marrow.Plays a role in hematopoiesis; required for normal numbers ofhematopoietic stem cells in bone marrow. Promotes in vitroosteoblast proliferation and differentiation (By similarity).Promotes neurite extension, axon growth and axon guidance; axonsgrow preferentially on surfaces that contain ALCAM (Bysimilarity). Mediates outgrowth and pathfinding for retinalganglion cell axons (By similarity)..
    Tissue Specificity Strongest expression in the lung, then brain,liver, and kidney. Present in the somatosensory system, basalganglia, cortex, olfactory system, and circumventricular organs.
    Subcellular Localization Cell membrane; Detected at the immunologicalsynapse, i.e, at the contact zone between antigen-presentingdendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3complex at the immunological synapse..
    Uniprot ID Alcam: O35112
    指标别名 CD166 antigen; Activated leukocyte cell adhesion molecule; HB2; KG-CAM; Protein MEMD; SB-10 antigen; CD166; Alcam;

    CD166 antigen指标简介

    CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)18972227412018302905
    Standard deviation9.2637.54158.9811.8650.63203.35
    CV(%)4.95.25.85.96.17.0

    Rat ALCAM ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30min)
    Concentration(pg/ml)078156312625125025005000
    O.D.0.0040.0640.1180.2150.4500.7981.5782.317
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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