|Product Name||Nitrotyrosine ELISA Kit|
|Description||Colorimetric detection of Nitrotyrosine. 96wells/kit, with removable strips.|
|Cite This Product||Nitrotyrosine ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7116)|
|Validated Species||All Animals|
|Cross Reactivity||There is no detectable cross-reactivity.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
|Assay Range||62.5 - 8000 nM|
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||Cell lysates, Plasma, Serum, Urine|
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Storage||Store at 4°C.|
|Nitrosylated BSA Coated Plate||1 Plate|
|Nitrotyrosine Standard||1 vial/110?l|
|Nitrotyrosine HRP Conjugated Monoclonal Antibody||1 vial/75?l|
|Sample and Standard Diluent (Red)||1 vial/50mL|
|Nitrotyrosine Antibody Diluent (Blue)||1 vial/13mL|
|Wash Buffer Concentrate (10X)||1 vial/ 50mL|
|TMB Substrate||1 vial/ 13 ml|
|Stop Solution||1 vial/ 13 ml|
Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay)–EK7116 Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 62.5 – 8000 nM.
Diagram of the Competitive ELISA
Diagram of the Preparation of Nitrotyrosine Standards
Diagram of the Triplicate Sample Plate Format