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Mouse BMP-2 ELISA Kit

BDA2|BMP-2|BMP2|BMP 2|BMP-2A|Bmp2|BMP2_HUMAN|BMP2A|Bone morphogenetic protein 2|Bone morphogenetic protein 2A

分类:

ELISA试剂盒 - Mouse Elisa Kit

说明书:

EK0313

2000元/96T  

CCS/S/BT

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称Mouse BMP-2 ELISA Kit
    产品描述Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-2
    引用此产品Mouse BMP-2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK0313)
    验证种属Mouse
    免疫原标准品表达系统:CHO,Q283-R396
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    规格96 wells/kit

    产品性质

    敏感性<2pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围31.2pg/ml-2000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型bone tissue, cell culture supernates and serum. *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体monoclonal antibody from rat
    检测抗体polyclonal antibody from goat
    一般表达浓度以下文章提供了BMP2的表达量的信息

    保存条件4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU名称数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- mouse BMP-2 antibody1
    ST0000-10lyophilized recombinant mouse BMP-2 standard10ng/tubex2
    AR1107biotinylated anti- mouse BMP-2 antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4



    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    1. 96孔读板仪器。

    2. 自动洗板机.

    3. 移液器和枪头。建议使用多通道移液器/排枪。

    4. Eppendorf 管。

    5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

    0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

    基因名BMP2
    蛋白名Bone morphogenetic protein 2
    基因全名Bone morphogenetic protein 2
    蛋白功能Induces cartilage and bone formation.
    Subcellular LocalizationSecreted.
    Uniprot IDBMP2: P21274
    指标别名Bone morphogenetic protein 2; BMP-2; Bone morphogenetic protein 2A; BMP-2A; Bmp2; Bmp-2;
    相关研究领域cardiovascular|angiogenesis|growth factors|tgf|signal transduction|growth factors/hormones|cytoskeleton / ecm|extracellular matrix|structures|bone|stem cells|signaling pathways|tgf beta|secreted|neural stem cells|extracellular|cancer

    Bone morphogenetic protein 2指标简介

    Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

    批内差/批间差


    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)5119880248221789
    Standard deviation2.1910.0934.482.5412.1532.34
    CV(%)4.35.14.35.35.54.1

    Mouse BMP-2 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20min)

    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.0050.0340.0590.1190.2770.6801.6422.417

    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。


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