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Mouse beta IG-H3/TGFBI ELISA Kit

Beta ig | Beta ig h3 | Beta ig-h3 | Big h3 | BIGH3 | CDB1 | CDG2 | CDGG1 | CSD | CSD1 | CSD2 | CSD3 | EBMD | Kerato epithelin | Kerato-epithelin | LCD1 | RGD CAP | RGD-CAP | TGFBI | Q15582| βIG-H3| bIG-H3

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ELISA试剂盒 - Mouse Elisa Kit

说明书:

EK1191

2000元/96T  

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Mouse beta IG-H3/TGFBI ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse beta IG-H3/TGFBI
    引用此产品 Mouse beta IG-H3/TGFBI ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1191)
    验证种属 Mouse
    免疫原 标准品表达系统:NSO, G24-H683
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <5pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 156pg/ml-10000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from rat
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了Tgfbi的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- mouse beta IG-H3 antibody1
    ST0000-10lyophilized recombinant mouse beta IG-H3 standard10ng/tube x 2
    AR1107biotinylated anti- mouse beta IG-H3 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 Tgfbi
    蛋白名 Transforming growth factor-beta-induced protein ig-h3
    基因全名 Transforming growth factor-beta-induced protein ig-h3
    蛋白功能 Plays a role in cell adhesion (PubMed:8024701). May playa role in cell-collagen interactions (By similarity)..
    Tissue Specificity Expressed in heart, kidney, liver, skeletalmuscle, testis, thyroid and uterus (PubMed:8024701)..
    Subcellular Localization Secreted.Secreted, extracellular space, extracellular matrix. May be associated both withmicrofibrils and with the cell surface..
    Uniprot ID Tgfbi: P82198
    指标别名 Transforming growth factor-beta-induced protein ig-h3; Beta ig-h3; Tgfbi;
    相关研究领域 signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|collagen|growth factors/hormones|tgf|structures|bone|neuroscience|sensory system|visual system ecm proteins|developmental biology|organogenesis|excretory system development|kidney development

    Transforming growth factor-beta-induced protein ig-h3指标简介

    Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)22199128462958884339
    Standard deviation13.4851.53139.4512.6848.84269.01
    CV(%)6.15.24.94.35.56.2

    Mouse beta IG-H3/TGFBI ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20 min)
    Concentration(pg/ml)015631262512502500500010000
    O.D.0.0040.0360.0710.1470.3210.7331.3732.509
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。


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