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Mouse BAFF ELISA Kit

B lymphocyte stimulator| B-cell-activating factor| BAFF| BLyS| CD257| Dendritic cell-derived TNF-like molecule| soluble form| TALL-1| TN13B_HUMAN| TNF- and APOL-related leukocyte expressed ligand 1|

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ELISA试剂盒 - Mouse Elisa Kit

说明书:

EK0664

2000元/96T  

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    产品名称 Mouse BAFF ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BAFF
    引用此产品 Mouse BAFF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK0664)
    验证种属 Mouse
    免疫原 标准品表达系统:NSO,A127-L309
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 46.9pg/ml-3000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from rat
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了TNFSF13B的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- mouse BAFF antibody1
    ST0000-10lyophilized recombinant mouse BAFF standard10ng/tubex2
    AR1107biotinylated anti- mouse BAFF antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 TNFSF13B
    蛋白名 Tumor necrosis factor ligand superfamily member 13B
    基因全名 tumor necrosis factor (ligand) superfamily, member 13b
    Uniprot ID TNFSF13B: Q3KP92
    指标别名 Tumor necrosis factor (Ligand) superfamily, member 13b; Tumor necrosis factor (Ligand) superfamily, member 13b, isoform CRA_d; Tnfsf13b; mCG_3400;
    相关研究领域 immunology|adaptive immunity|b cells|non-cd|innate immunity|cytokines|tnf superfamily|signal transduction|growth factors/hormones|tnf|cancer|growth factors|kits/ lysates/ other|kits|elisa kits|tnf elisa kits

    tumor necrosis factor (ligand) superfamily, member 13b指标简介

    B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)13931215081453541397
    Standard deviation4.911.558.814.433.6124.3
    CV(%)3.53.73.99.99.58.9

    Mouse BAFF ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20min)
    Concentration(pg/ml)046.99418837575015003000
    O.D.0.0780.1470.2010.3150.5070.8911.5162.346
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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