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Mouse Angiopoietin-1 ELISA Kit

1110046O21Rik| AGP 1| AGP1| AGPT| ANG 1| ANG1| Angiopoietin1| ANGPT 1| ANGPT1| KIAA0003


ELISA试剂盒 - Mouse Elisa Kit











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Product Brief

  • 产品概况

    产品名称 Mouse Angiopoietin-1 ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Angiopoietin-1
    引用此产品 Mouse Angiopoietin-1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1296)
    验证种属 Mouse
    免疫原 标准品表达系统:NSO,H16-F497
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit


    敏感性 <10pg/ml
    检测范围 156pg/ml-10,000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from rat
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了ANGPT1的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)


    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- mouse Angiopoietin-1 antibody1
    ST0000-10lyophilized recombinant mouse Angiopoietin-1 standard10ng/tubex2
    AR1107biotinylated anti- mouse Angiopoietin-1 antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4


    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 ANGPT1
    蛋白名 Angiopoietin-1
    基因全名 Angiopoietin-1
    蛋白功能 Binds and activates TEK/TIE2 receptor by inducing itsdimerization and tyrosine phosphorylation. Plays an important rolein the regulation of angiogenesis, endothelial cell survival,proliferation, migration, adhesion and cell spreading,reorganization of the actin cytoskeleton, but also maintenance ofvascular quiescence. Required for normal angiogenesis and heartdevelopment during embryogenesis. After birth, activates orinhibits angiogenesis, depending on the context. Inhibitsangiogenesis and promotes vascular stability in quiescent vessels,where endothelial cells have tight contacts. In quiescent vessels,ANGPT1 oligomers recruit TEK to cell-cell contacts, formingcomplexes with TEK molecules from adjoining cells, and this leadsto preferential activation of phosphatidylinositol 3-kinase andthe AKT1 signaling cascades. In migrating endothelial cells thatlack cell-cell adhesions, ANGT1 recruits TEK to contacts with theextracellular matrix, leading to the formation of focal adhesioncomplexes, activation of PTK2/FAK and of the downstream kinasesMAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation ofsprouting angiogenesis. Mediates blood vesselmaturation/stability. Implicated in endothelial developmentalprocesses later and distinct from that of VEGF. Appears to play acrucial role in mediating reciprocal interactions between theendothelium and surrounding matrix and mesenchyme (By similarity)..
    Subcellular Localization Secreted.
    Uniprot ID ANGPT1: O08538
    指标别名 Angiopoietin-1; ANG-1; Angpt1; Agpt;
    相关研究领域 cardiovascular|angiogenesis|growth factors|angiopoietin


    Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.


    Intra-Assay PrecisionInter-Assay Precision
    Standard deviation0.0550.1310.3010.0860.1810.369

    Mouse Angiopoietin-1 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30min)






0.01M TBS洗涤3次。


0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。


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