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Mouse ALK-1/ACVRL1 ELISA Kit

Activin A receptor | ACVRL1 | ACVRLK1 | ALK-1 | ALK1 | ALK 1 | HHT | HHT2 | ORW2 | SKR3 | TSR-I | TSRI | Q61288

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ELISA试剂盒 - Mouse Elisa Kit

说明书:

EK1519

2000元/96T

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    产品名称 Mouse ALK-1/ACVRL1 ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ALK-1/ACVRL1
    引用此产品 Mouse ALK-1/ACVRL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1519)
    验证种属 Mouse
    免疫原 标准品表达系统:NSO,D23-P119
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <15pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 46.9pg/ml-3000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    一般表达浓度 以下文章提供了Acvrl1的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- mouse ALK-1 antibody1
    ST0000-10Lyophilized recombinant mouse ALK-1 standard10ng/tube??2
    AR1107Biotinylated anti- mouse ALK-1 antibody130??l(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130??l(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 Acvrl1
    蛋白名 Serine/threonine-protein kinase receptor R3
    基因全名 Serine/threonine-protein kinase receptor R3
    蛋白功能 Type I receptor for TGF-beta family ligands BMP9/GDF2and BMP10 and important regulator of normal blood vesseldevelopment. On ligand binding, forms a receptor complexconsisting of two type II and two type I transmembraneserine/threonine kinases. Type II receptors phosphorylate andactivate type I receptors which autophosphorylate, then bind andactivate SMAD transcriptional regulators. May bind activin aswell..
    Subcellular Localization Cell membrane; Single-pass type I membraneprotein.
    Uniprot ID Acvrl1: Q61288
    指标别名 Serine/threonine-protein kinase receptor R3; SKR3; 2.7.11.30; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I; Acvrl1; Acvrlk1, Alk-1;
    相关研究领域 cardiovascular|angiogenesis|growth factors|tgf|signal transduction|metabolism|amino acids|protein phosphorylation|ser / thr kinases kinases|growth factors/hormones|vasculature|endothelium|pathways and processes|metabolic signaling pathways|amino acid metabolism|types of disease|cancer

    Serine/threonine-protein kinase receptor R3指标简介

    Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)16358215071896541620
    Standard deviation10.2731.43108.513.0447.74123.12
    CV(%)6.35.47.26.97.37.6

    Mouse ALK-1/ACVRL1 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30min)
    Concentration(pg/ml)046.993.8187.537575015003000
    O.D.0.0320.0600.1380.2390.5381.0451.8952.280
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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