|Product Name||Goat Anti-Rabbit IgG（H+L） Secondary Antibody, Unconjugated|
|Description||This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.|
|Cite This Product||Goat Anti-Rabbit IgG Secondary Antibody, Unconjugated (Boster Biological Technology, Wuhan, CHINA, Catalog # BA1039)|
|Application||ELISA, IF, IHC, ICC, WB|
|Immunogen||Rabbit IgG (whole molecule).|
|Storage||At -20°C for 2 years. Avoid repeated freezing and thawing.|
Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.
试剂稀释：具体浓度由用户自己确定。0.01M PBS 缓冲液(PH7.0-7.6)，或1%乙酸钠稀释均可。稀释后不能长期保存。加入防腐剂（如0.05%NaN3）和试剂级BSA 至1%后，抗体可以长期保存。对抗体进行标记时，如HRP、FITC、BIOTIN 等，试剂中不能含有BSA 和NaN3。