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Human STC2 PicoKine ELISA Kit

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1988

2800元/96T  

ELISA试剂盒(96T)

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Product Brief

  • 产品概况

    产品名称Human STC2 PicoKine ELISA Kit
    产品描述For quantitative detection of human STC2 in cell culture supernates, serum.
    验证种属Human
    免疫原标准品表达系统:NSO, T25-R302
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    规格1x96 well plate

    产品性质

    敏感性<5pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围7.8pg/ml-500pg/ml*检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型cell culture supernates, serum. *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
    检测抗体
    一般表达浓度以下文章提供了STC2的表达量的信息:
      保存条件4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

      指标相关信息 (来源: Uniprot.org)

      小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
      基因名STC2
      蛋白名Stanniocalcin-2
      基因全名stanniocalcin 2
      Tissue SpecificityNatural and recombinant human STC2
      Uniprot IDSTC2: O76061
      指标别名Stanniocalcin-2; STC-2; Stanniocalcin-related protein; STC-related protein; STCRP; STC2

      stanniocalcin 2指标简介

      Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 5q35.2. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

      批内差/批间差

      Intra-Assay PrecisionInter-Assay Precision

      Human STC2 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

      (TMB 孵育条件: 37°C, 15-20)Concentration(pg/ml)|0|7.8|15.6|31.2|62.5|125|250|500O.D.|0.033|0.082|0.135|0.214|0.402|0.731|1.288|2.162
      *典型数据仅供参考。用户需要自己建立标准曲线。

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