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Human Serpin A12 PicoKine ELISA Kit

Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1874

2800元/96T  

cell culture supernates,serum and plasma (heparin;EDTA) and urine.

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    货号EK1874
    产品名称Human Serpin A12 PicoKine ELISA Kit
    产品描述For quantitative detection of human Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA) and urine.
    免疫原NSO, L21-K414
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    敏感性<50pg/ml
    检测范围156pg/ml-10000pg/ml
    基因名称SERPINA12
    基因全名serpin family A member 12
    Uniprot IDQ8IW75
    指标别名Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12
    背景资料This gene is mapped to 14q32.13.
    显色时间20-25

    试剂盒内容

    SKU名称数量
    Catalog numberDescriptionQuantity
    EK1874-CAP96-well plate precoated with anti- human Serpin A12 antibody1
    EK1874-STLyophilized recombinant human Serpin A12 standard10ng/tube×2
    EK1874-DABiotinylated anti- human Serpin A12 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)2121109546523212356154
    Standard deviation11.2468.76245.9310.9079.04332.32
    CV(%)5.3%6.2%4.5%4.7%6.4%5.4%

    Human Serpin A12 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25)
    Concentration(pg/ml)015631262512502500500010000
    O.D.0.0250.1600.2630.4910.8521.2901.8782.305
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

1:准备试剂和标准品

2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。

3:每孔添加100ul生物素标记抗体,37℃反应60分钟。

4:0.01M TBS洗涤3次。

5:每孔添加100ulABC,37℃反应30分钟。

6:0.01M TBS洗涤5次。

7:TMB37℃避光反应30分钟以内。

8:加入TMB终止液,读数。


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