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Human Nectin-3/CD113 PicoKine ELISA Kit

nectin cell adhesion molecule 3|Nectin-3|Nectin-3; CDw113; Nectin cell adhesion molecule 3; Poliovirus receptor-related protein 3; CD113; NECTIN3; PRR3; PVRL3

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1961

2800元/96T  

cell culture supernates, serum and plasma(heparin, EDTA).

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称Human Nectin-3/CD113 PicoKine ELISA Kit
    产品描述For quantitative detection of human Nectin-3/CD113 in cell culture supernates, serum and plasma(heparin, EDTA).
    引用此产品Human Nectin-3/CD113 PicoKine ELISA Kit(Boster Biological Technology, Wuhan, China. Catalog #EK1961)
    验证种属Human
    免疫原标准品表达系统:NSO, G58-T404
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    规格96 wells/kit

    产品性质

    敏感性<20pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围125pg/ml-8000pg/ml*检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型cell culture supernates, serum and plasma(heparin, EDTA).*对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体A monoclonal antibody from mouse
    检测抗体a biotinylated detection polyclonal antibody from goat
    一般表达浓度以下文章提供了NECTIN3的表达量的信息:
      保存条件4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

      试剂盒内容

      SKU名称数量
      Catalog numberDescriptionQuantity
      EK1961-CAP96-well plate precoated with anti- human Nectin-3/CD113 antibody1
      EK1961-STLyophilized recombinant human Nectin-3/CD113 standard10ng/tube×2
      EK1961-DABiotinylated anti- human Nectin-3/CD113 antibody130ul(dilution 1:100)
      AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
      AR1106-1Sample diluent buffer30 ml
      AR1106-2Antibody diluent buffer12ml
      AR1106-3ABC diluent buffer12ml
      AR1104TMB color developing agent10ml
      AR1105TMB stop solution10ml
      PLA-SEAAdhesive cover4
      *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

      需要用户自己提供的器材和试剂

      • 1.96孔读板仪器。
      • 2.自动洗板机.
      • 3.移液器和枪头。建议使用多通道移液器/排枪。
      • 4.Eppendorf 管。
      • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
        • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
        • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      *样品准备的相关试剂也不含在试剂盒中。

      指标相关信息 (来源: Uniprot.org)

      小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
      基因名NECTIN3
      蛋白名Nectin-3
      基因全名nectin cell adhesion molecule 3
      Uniprot IDNECTIN3: Q9NQS3
      指标别名Nectin-3; CDw113; Nectin cell adhesion molecule 3; Poliovirus receptor-related protein 3; CD113; NECTIN3; PRR3; PVRL3

      nectin cell adhesion molecule 3指标简介

      Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. It is mapped to 19q13.32. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.

      批内差/批间差

      Intra-Assay PrecisionInter-Assay Precision
      Sample123123
      n161616242424
      Mean(pg/ml)15987338092399393980
      Standard deviation10.6545.40217.1115.0650.71163.18
      CV(%)6.7%5.2%5.7%6.3%5.4%4.1%

      Human Nectin-3/CD113 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

      (TMB 孵育条件: 37°C, 15-20)
      Concentration(pg/ml)01252505001000200040008000
      O.D.0.1100.1830.2300.3860.5960.9911.6612.295
      *典型数据仅供参考。用户需要自己建立标准曲线。

    Instructions

    典型操作程序:

    1:准备试剂和标准品

    2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。

    3:每孔添加100ul生物素标记抗体,37℃反应60分钟。

    4:0.01M TBS洗涤3次。

    5:每孔添加100ulABC,37℃反应30分钟。

    6:0.01M TBS洗涤5次。

    7:TMB37℃避光反应30分钟以内。

    8:加入TMB终止液,读数。

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