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Human Myostatin/GDF8 PicoKine ELISA Kit

Growth/differentiation factor 8; GDF-8; Myostatin; MSTN; GDF8

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1265

2800元/96T  

cell culture supernates,tissue,serum and plasma(heparin;EDTA).

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    货号EK1265
    产品名称Human Myostatin/GDF8 PicoKine ELISA Kit
    产品描述For quantitative detection of human Myostatin/GDF8 in cell culture supernates, tissue, serum and plasma(heparin, EDTA).
    免疫原NSO, D267-S375
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    敏感性<10pg/ml
    检测范围31.2pg/ml-2000pg/ml
    基因名称MSTN
    基因全名myostatin
    Uniprot IDO14793
    指标别名Growth/differentiation factor 8; GDF-8; Myostatin; MSTN; GDF8
    背景资料Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 2q32.2. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
    显色时间15-20

    试剂盒内容

    SKU名称数量
    Catalog numberDescriptionQuantity
    EK1265-CAP96-well plate precoated with anti- human Myostatin/GDF8 antibody1
    EK1265-STLyophilized recombinant human Myostatin/GDF8 standard10ng/tube×2
    EK1265-DABiotinylated anti- human Myostatin/GDF8 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)5117162861181638
    Standard deviation2.1410.4328.263.2913.2142.11
    CV(%)4.2%6.1%4.5%5.4%7.3%6.6%

    Human Myostatin/GDF8 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.0760.1500.1850.2920.5200.8391.4962.292
    *典型数据仅供参考。用户需要自己建立标准曲线。

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