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Human Myostatin/GDF8 PicoKine ELISA Kit

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1265

2800元/96T  

ELISA试剂盒(96T)

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Product Brief

  • 产品概况

    产品名称Human Myostatin/GDF8 PicoKine ELISA Kit
    产品描述For quantitative detection of human Myostatin/GDF8 in cell culture supernates, tissue, serum and plasma(heparin, EDTA).
    验证种属Human
    免疫原标准品表达系统:NSO, D267-S375
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    规格1x96 well plate

    产品性质

    敏感性<10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围15.6pg/ml-1000pg/ml*检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型cell culture supernates, tissue, serum and plasma(heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
    检测抗体
    一般表达浓度以下文章提供了MSTN的表达量的信息:
      保存条件4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

      指标相关信息 (来源: Uniprot.org)

      小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
      基因名MSTN
      蛋白名Growth/differentiation factor 8
      基因全名myostatin
      Tissue SpecificityNatural and recombinant human Myostatin/GDF8
      Uniprot IDMSTN: O14793
      指标别名Growth/differentiation factor 8; GDF-8; Myostatin; MSTN; GDF8

      myostatin指标简介

      Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 2q32.2. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

      批内差/批间差

      Intra-Assay PrecisionInter-Assay Precision

      Human Myostatin/GDF8 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

      (TMB 孵育条件: 37°C, 15-20)Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000O.D.|0.076|0.150|0.185|0.292|0.520|0.839|1.496|2.292
      *典型数据仅供参考。用户需要自己建立标准曲线。

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