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Human FLRT2 PicoKine ELISA Kit

Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; FLRT2; KIAA0405; UNQ232/PRO265

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1971

2800元/96T  

cell culture supernates,serum and plasma (heparin;EDTA).

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    货号EK1971
    产品名称Human FLRT2 PicoKine ELISA Kit
    产品描述For quantitative detection of human FLRT2 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原NSO, C36-T660
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    敏感性<10pg/ml
    检测范围31.2pg/ml-2000pg/ml
    基因名称FLRT2
    基因全名fibronectin leucine rich transmembrane protein 2
    Uniprot IDO43155
    指标别名Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; FLRT2; KIAA0405; UNQ232/PRO265
    背景资料Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 14q31.3. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.
    显色时间20-25

    试剂盒内容

    SKU名称数量
    Catalog numberDescriptionQuantity
    EK1971-CAP96-well plate precoated with anti- human FLRT2 antibody1
    EK1971-STLyophilized recombinant human FLRT2 standard10ng/tube×2
    EK1971-DABiotinylated anti- human FLRT2 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)4523784954246915
    Standard deviation2.1613.5156.032.7512.0558.56
    CV(%)4.8%5.7%6.6%5.1%4.9%6.4%

    Human FLRT2 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25)
    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.1000.1910.2680.3900.8671.0721.6152.137
    *典型数据仅供参考。用户需要自己建立标准曲线。

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