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Human Cystatin D/CST5 PicoKine ELISA Kit

Cystatin-D| Cystatin-5| CST5

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1964

2800元/96T  

CCS/S/P

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    产品名称Human Cystatin D/CST5 PicoKine ELISA Kit
    产品描述For quantitative detection of human Cystatin D/CST5 in cell culture supernates, serum and plasma (heparin, EDTA), saliva and urine.
    验证种属Human
    免疫原标准品表达系统:E.coli, G21-V142
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    规格1x96 well plate

    产品性质

    敏感性<10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围15.6pg/ml-1000pg/ml*检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型cell culture supernates, serum and plasma (heparin, EDTA), saliva and urine. *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
    检测抗体
    一般表达浓度以下文章提供了CST5的表达量的信息:
      保存条件4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

      指标相关信息 (来源: Uniprot.org)

      小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
      基因名CST5
      蛋白名Cystatin-D
      基因全名cystatin D
      Tissue SpecificityNatural and recombinant human Cystatin D/CST5
      Uniprot IDCST5: P28325
      指标别名Cystatin-D; Cystatin-5; CST5

      cystatin D指标简介

      Cystatin-D is a protein that in humans is encoded by the CST5 gene. It is mapped to 20p11.21. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity.

      批内差/批间差

      Intra-Assay PrecisionInter-Assay Precision

      Human Cystatin D/CST5 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

      (TMB 孵育条件: 37°C, 15-20)Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000O.D.|0.013|0.063|0.113|0.208|0.361|0.713|1.211|2.038
      *典型数据仅供参考。用户需要自己建立标准曲线。

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