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Human CNDP1 PicoKine ELISA Kit

Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1957

2800元/96T  

cell culture supernates,serum and plasma (heparin;EDTA).

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    货号EK1957
    产品名称Human CNDP1 PicoKine ELISA Kit
    产品描述For quantitative detection of human CNDP1 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原NSO, S27-H507
    交叉反应There is no detectable cross-reactivity with other relevant proteins.
    敏感性<50pg/ml
    检测范围312pg/ml-20000pg/ml
    基因名称CNDP1
    基因全名carnosine dipeptidase 1
    Uniprot IDQ96KN2
    指标别名Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380
    背景资料Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
    显色时间15-20

    试剂盒内容

    SKU名称数量
    Catalog numberDescriptionQuantity
    EK1957-CAP96-well plate precoated with anti- human CNDP1 antibody1
    EK1957-STLyophilized recombinant human CNDP1 standard20ng/tube×2
    EK1957-DABiotinylated anti- human CNDP1 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)6132702885562029189790
    Standard deviation30.04143.21602.1444.02131.31528.66
    CV(%)4.9%5.3%6.8%7.1%4.5%5.4%

    Human CNDP1 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml)03126251250250050001000020000
    O.D.0.1190.1630.2040.2740.4600.7481.2602.188
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

1:准备试剂和标准品

2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。

3:每孔添加100ul生物素标记抗体,37℃反应60分钟。

4:0.01M TBS洗涤3次。

5:每孔添加100ulABC,37℃反应30分钟。

6:0.01M TBS洗涤5次。

7:TMB37℃避光反应30分钟以内。

8:加入TMB终止液,读数。


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