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Human CA12 PicoKine ELISA Kit

carbonic anhydrase 12|Carbonic anhydrase 12|Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3; CA12

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1942

2800元/96T  

cell culture supernates, serum.

ELISA试剂盒(96T)

现货

选择规格:

总价格:

---元*1

选择数量:

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Product Brief

  • 产品概况

    产品名称 Human CA12 PicoKine ELISA Kit
    产品描述 For quantitative detection of human CA12 in cell culture supernates, serum.
    引用此产品 Human CA12 PicoKine ELISA Kit(Boster Biological Technology, Wuhan, China. Catalog #EK1942)
    验证种属 Human
    免疫原 标准品表达系统:NSO, A25-A354
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 31.2pg/ml-2000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, serum. *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 A monoclonal antibody from mouse
    检测抗体 a biotinylated detection polyclonal antibody from goat
    一般表达浓度 以下文章提供了CA12的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    EK1942-CAP96-well plate precoated with anti- human CA12 antibody1
    EK1942-STLyophilized recombinant human CA12 standard10ng/tube×2
    EK1942-DABiotinylated anti- human CA12 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 CA12
    蛋白名 Carbonic anhydrase 12
    基因全名 carbonic anhydrase 12
    Uniprot ID CA12: O43570
    指标别名 Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3; CA12

    carbonic anhydrase 12指标简介

    Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 15q22.2. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)5721788660231927
    Standard deviation3.198.9046.962.8814.3250.99
    CV(%)5.6%4.1%5.3%4.8%6.2%5.5%

    Human CA12 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.0300.0850.1380.2290.4230.8061.3912.271
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

1:准备试剂和标准品

2:每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

3:每孔添加100μl生物素标记抗体,37℃反应60分钟。

4:0.01M TBS洗涤3次。

5:每孔添加100μlABC,37℃反应30分钟。

6:0.01M TBS洗涤5次。

7:TMB37℃避光反应30分钟以内。

8:加入TMB终止液,读数。

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