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Human ALCAM ELISA Kit

ALCAM protein | CD166 antigen | Protein MEMD | Q13740

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK0995

2000元/96T

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Human ALCAM ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ALCAM
    引用此产品 Human ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK0995)
    验证种属 Human
    免疫原 标准品表达系统:NSO,W28-A526
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 312pg/ml-20,000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from mouse
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了ALCAM的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- human ALCAM antibody1
    ST0000-20lyophilized recombinant human ALCAM standard20ng/tubex2
    AR1107biotinylated anti- human ALCAM antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 ALCAM
    蛋白名 CD166 antigen
    基因全名 CD166 antigen
    蛋白功能 Cell adhesion molecule that binds to CD6. Involved inneurite extension by neurons via heterophilic and homophilicinteractions. May play a role in the binding of T- and B-cells toactivated leukocytes, as well as in interactions between cells ofthe nervous system.
    Tissue Specificity Spleen, placenta, liver, and weakly in liver.Expressed by activated T-cells, B-cells, monocytes and thymicepithelial cells. Expressed by neurons in the brain. Restrictedexpression in tumor cell lines. Preferentially expressed in highlymetastasizing melanoma cell lines..
    Sequence Similarities Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Subcellular Localization Membrane; Single-pass type I membraneprotein.
    Uniprot ID ALCAM: Q13740
    指标别名 CD166 antigen; Activated leukocyte cell adhesion molecule; CD166; ALCAM; MEMD;
    相关研究领域 immunology|adaptive immunity|b cells|cd|t cells|kits/ lysates/ other|kits|elisa kits|cd markers elisa kits

    CD166 antigen指标简介

    CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(ng/ml)3.076.4213.263.587.0414.98
    Standard deviation0.1500.3270.7560.2180.4440.959
    CV(%)4.95.15.76.16.36.4

    Human ALCAM ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30min)
    Concentration(pg/ml)03126251250250050001000020000
    O.D.0.0330.1090.1370.2150.3850.6881.1382.063
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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