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Human AGRP/agouti-related ELISA Kit

Agrp|AGRT|ART|ASIP2

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1427

2000元/96T

CCS/S/P/U

ELISA试剂盒(96T)

现货

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Product Brief

  • 产品概况

    产品名称 Human AGRP/agouti-related ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human AGRP/agouti-related
    引用此产品 Human AGRP/agouti-related ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1427)
    验证种属 Human
    免疫原 标准品表达系统:sf21,A21-T132
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <4pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 7.8pg/ml-500pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    一般表达浓度 以下文章提供了AGRP的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- human AGRP antibody1
    ST0000-10Lyophilized recombinant human AGRP standard10ng/tube x 2
    AR1107Biotinylated anti- human AGRP antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 AGRP
    蛋白名 Agouti-related protein
    基因全名 Agouti-related protein
    蛋白功能 Plays a role in weight homeostasis. Involved in thecontrol of feeding behavior through the central melanocortinsystem. Acts as alpha melanocyte-stimulating hormone antagonist byinhibiting cAMP production mediated by stimulation of melanocortinreceptors within the hypothalamus and adrenal gland. Has very lowactivity with MC5R (By similarity). Is an inverse agonist for MC3Rand MC4R being able to suppress their constitutive activity. Itpromotes MC3R and MC4R endocytosis in an arrestin-dependentmanner..
    Tissue Specificity Expressed primarily in the adrenal gland,subthalamic nucleus, and hypothalamus, with a lower level ofexpression occurring in testis, lung, and kidney.
    Subcellular Localization Secreted.Golgi apparatus lumen.
    Uniprot ID AGRP: O00253
    指标别名 Agouti-related protein; AGRP; AGRT, ART;
    相关研究领域 signal transduction|signaling pathway|calcium signaling|neuroscience|neurology process|metabolism|endocrine system|hypothalamic pituitary adrenal axis|cardiovascular|lipids / lipoproteins|fatty acids|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|obesity|cancer|redox metabolism|fatty acid oxidation

    Agouti-related protein指标简介

    Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)42.715534750.8192420
    Standard deviation1.847.6019.433.0510.7526.88
    CV(%)4.34.95.66.05.66.4

    Human AGRP/agouti-related ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20min)
    Concentration(pg/ml)07.815.631.362.5125250500
    O.D.0.0120.0650.1130.2230.4400.8141.4392.332
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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