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Human ADAMTS1 ELISA Kit

A disintegrin and metalloproteinase with thrombospondin motifs 1|A disintegrin like and metalloprotease with thrombospondin type 1 motif 1|A disintegrin-like and metalloprotease (reprolysin type) with

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1340

2000元/96T

CCS/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Human ADAMTS1 ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAMTS1
    引用此产品 Human ADAMTS1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1340)
    验证种属 Human
    免疫原 标准品表达系统:NSO,F253-A734
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 93.8pg/ml-6000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, serum and plasma(heparin). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from mouse
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了ADAMTS1的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- human ADAMTS1 antibody1
    ST0000-10lyophilized recombinant human ADAMTS1 standard10ng/tubex2
    AR1107biotinylated anti- human ADAMTS1 antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 ADAMTS1
    蛋白名 A disintegrin and metalloproteinase with thrombospondin motifs 1
    基因全名 A disintegrin and metalloproteinase with thrombospondin motifs 1
    蛋白功能 Cleaves aggrecan, a cartilage proteoglycan, and may beinvolved in its turnover (By similarity). Has angiogenic inhibitoractivity. Active metalloprotease, which may be associated withvarious inflammatory processes as well as development of cancercachexia. May play a critical role in follicular rupture..
    Sequence Similarities Contains 1 disintegrin domain.
    Subcellular Localization Secreted, extracellular space, extracellularmatrix.
    Uniprot ID ADAMTS1: Q9UHI8
    指标别名 A disintegrin and metalloproteinase with thrombospondin motifs 1; ADAM-TS 1; ADAM-TS1; ADAMTS-1; 3.4.24.-; METH-1; ADAMTS1; KIAA1346, METH1;
    相关研究领域 cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp|tags & cell markers|cell type markers|tumor associated|signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|cancer|invasion/microenvironment|adam protein family|cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adam ts

    A disintegrin and metalloproteinase with thrombospondin motifs 1指标简介

    ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What?€?s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)120827414137185030354726
    Standard deviation42.28117.9202.798.05176.03302.5
    CV(%)3.54.34.95.35.86.4

    Human ADAMTS1 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25min)
    Concentration(pg/ml)093.7187.5375750150030006000
    O.D.0.0260.0960.1600.2780.5130.9201.5992.476
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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