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Human ADAM9 ELISA Kit

ADAM 9|ADAM metallopeptidase domain 9|Adam9|ADAM9_HUMAN|Cellular disintegrin-related protein|Cone rod dystrophy 9|CORD9|Disintegrin and metalloproteinase domain-containing protein 9|MCMP|MDC9|Meltrin-

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1341

2000元/96T

CCS/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Human ADAM9 ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM9
    引用此产品 Human ADAM9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1341)
    验证种属 Human
    免疫原 标准品表达系统:NSO,A29-D697
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <10pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 15.6pg/ml-1000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, serum and plasma(heparin). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from mouse
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了ADAM9的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- human ADAM9 antibody1
    ST0000-10lyophilized recombinant human ADAM9 standard10ng/tubex2
    AR1107biotinylated anti- human ADAM9 antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml-Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 ADAM9
    蛋白名 Disintegrin and metalloproteinase domain-containing protein 9
    基因全名 Disintegrin and metalloproteinase domain-containing protein 9
    蛋白功能 Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activityfor APP..
    Tissue Specificity Widely expressed. Expressed in chondrocytes.Isoform 2 is highly expressed in liver and heart..
    Sequence Similarities Contains 1 disintegrin domain.
    Subcellular Localization Isoform 1: Cell membrane; Single-pass type Imembrane protein.
    Uniprot ID ADAM9: Q13443
    指标别名 Disintegrin and metalloproteinase domain-containing protein 9; ADAM 9; 3.4.24.-; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase; ADAM9; KIAA0021, MCMP, MDC9, MLTNG;
    相关研究领域 signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|mmp|adam protein family|cancer|invasion/microenvironment|cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adams

    Disintegrin and metalloproteinase domain-containing protein 9指标简介

    Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM("a disintegrin and metalloproteinase") family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)145363594176380658
    Standard deviation7.5420.6936.2311.4428.1251.98
    CV(%)5.25.76.16.57.47.9

    Human ADAM9 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20min)
    Concentration(pg/ml)015.631.262.51252505001000
    O.D.0.0420.0920.1210.1940.3190.5701.0232.010
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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