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Human ADAM15 ELISA Kit

ADAM15 | ADAM 15 | MDC15 | MDC 15 | MDC-15 | Metargidin | Q13444

分类:

ELISA试剂盒 - Human Elisa Kit

说明书:

EK1484

2000元/96T

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Human ADAM15 ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM15
    引用此产品 Human ADAM15 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1484)
    验证种属 Human
    免疫原 标准品表达系统:NSO,D207-T696
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <12pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 62.5pg/ml-4000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    一般表达浓度 以下文章提供了ADAM15的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- human ADAM15 antibody1
    ST0000-10Lyophilized recombinant human ADAM15 standard10ng/tube??2
    AR1107Biotinylated anti- human ADAM15 antibody130??l(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130??l(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 ADAM15
    蛋白名 Disintegrin and metalloproteinase domain-containing protein 15
    基因全名 Disintegrin and metalloproteinase domain-containing protein 15
    蛋白功能 Active metalloproteinase with gelatinolytic andcollagenolytic activity. Plays a role in the wound healingprocess. Mediates both heterotypic intraepithelial cell/T-cellinteractions and homotypic T-cell aggregation. Inhibits beta-1integrin-mediated cell adhesion and migration of airway smoothmuscle cells. Suppresses cell motility on or towards fibronectinpossibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cellsurface expression via ERK1/2 inactivation. Cleaves E-cadherin inresponse to growth factor deprivation. Plays a role in glomerularcell migration. Plays a role in pathological neovascularization.May play a role in cartilage remodeling. May be proteolyticallyprocessed, during sperm epididymal maturation and the acrosomereaction. May play a role in sperm-egg binding through itsdisintegrin domain..
    Tissue Specificity Expressed in colon and small intestine.Expressed in airway smooth muscle and glomerular mesangial cells(at protein level). Ubiquitously expressed. Overexpressed inatherosclerotic lesions. Constitutively expressed in culturedendothelium and smooth muscle. Expressed in chondrocytes.Expressed in airway smooth muscle and glomerular mesangial cells..
    Subcellular Localization Endomembrane system; The majority of the protein is localized in aperinuclear compartment which may correspond to the trans-Golginetwork or the late endosome. The pro-protein is the majordetectable form on the cell surface, whereas the majority of theprotein in the cell is processed (By similarity)..
    Uniprot ID ADAM15: Q13444
    指标别名 Disintegrin and metalloproteinase domain-containing protein 15; ADAM 15; 3.4.24.-; Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin; ADAM15; MDC15;
    相关研究领域 signal transduction|cytoskeleton / ecm|cell adhesion|integrins|alpha|beta|extracellular matrix|ecm enzymes|adam protein family|cancer|invasion/microenvironment|cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adams

    Disintegrin and metalloproteinase domain-containing protein 15指标简介

    Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)3661287245341213392874
    Standard deviation27.81113.25181.5235.43123.19267.28
    CV(%)7.68.87.48.69.29.3

    Human ADAM15 ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20min)
    Concentration(pg/ml)062.5125250500100020004000
    O.D.0.0610.0830.1090.1850.4040.6641.3732.079
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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