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Human A2M/alpha2-Macroglobulin ELISA Kit

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|A2m|A2MD|A2MG_HUMAN|Alpha-2-M|Alpha-2-macroglobulin|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|CPAMD5|FWP007|Pr

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ELISA试剂盒 - Human Elisa Kit

说明书:

EK1118

2000元/96T

CCS/CL/S/P

ELISA试剂盒(96T)

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 Human A2M/Alpha2-Macroglobulin ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human A2M/alpha2-Macroglobulin
    引用此产品 Human A2M/Alpha2-Macroglobulin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog #EK1118)
    验证种属 Human
    免疫原 标准品表达系统:from human plasma
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    规格 96 wells/kit

    产品性质

    敏感性 <20pg/ml
    *敏感性/最低检测浓度计算方式:敏感性等于20个空白对照孔的平均O.D.值加上2个标准差后对应的浓度。样品的O.D.读数只有在高过敏感性指标的时候才具有统计学显著性。
    检测范围 625pg/ml-40,000pg/ml *检测范围不代表敏感性。检测范围是以常见样品的指标表达量为参照,在博士德内部质检过程中使用的标准曲线区间。如果用户需要,可以加大标准曲线的初始稀释度来让试剂盒检测更低的范围。更多实验小贴士参见博士德的技术资料中心
    样品类型 cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). *对于上述未提及的样品类型,在一般情况下博士德的ELISA试剂盒可以检测。由于未检测的样品可能出现表达量过低的情况,建议先进行小规模实验验证可行性。
    包被抗体 monoclonal antibody from mouse
    检测抗体 polyclonal antibody from goat
    一般表达浓度 以下文章提供了A2M的表达量的信息:
    保存条件 4℃六个月,-20℃一年。避免不必要的重复溶解、冻结(加冰邮寄)

    试剂盒内容

    SKU 名称 数量
    Catalog numberDescriptionQuantity
    -96-well plate precoated with anti- human A2M antibody1
    ST0000-40lyophilized human A2M standard40ng/tubex2
    AR1107biotinylated anti- human A2M antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
    AR1106-1sample diluent buffer30 ml
    AR1106-2antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    -Adhesive cover4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1. 96孔读板仪器。
    • 2. 自动洗板机.
    • 3. 移液器和枪头。建议使用多通道移液器/排枪。
    • 4. Eppendorf 管。
    • 5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 A2M
    蛋白名 Alpha-2-macroglobulin
    基因全名 Alpha-2-macroglobulin
    蛋白功能 Is able to inhibit all four classes of proteinases by aunique "trapping" mechanism. This protein has a peptide stretch,called the "bait region" which contains specific cleavage sitesfor different proteinases. When a proteinase cleaves the baitregion, a conformational change is induced in the protein whichtraps the proteinase. The entrapped enzyme remains active againstlow molecular weight substrates (activity against high molecularweight substrates is greatly reduced). Following cleavage in thebait region a thioester bond is hydrolyzed and mediates thecovalent binding of the protein to the proteinase.
    Tissue Specificity Secreted in plasma..
    Sequence Similarities Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.
    Subcellular Localization Secreted.
    Uniprot ID A2M: P01023
    指标别名 Alpha-2-macroglobulin; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; A2M; CPAMD5; FWP007;
    相关研究领域 immunology|innate immunity|complement|cell biology|proteolysis / ubiquitin|protease inhibitors protease inhibitors

    Alpha-2-macroglobulin指标简介

    Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique "trapping" mechanism. This protein has a peptide stretch, called the "bait region" which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

    批内差/批间差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(ng/ml)6.1812.0425.216.7213.6826.81
    Standard deviation0.3770.7710.1740.4570.9852.12
    CV(%)6.16.46.96.87.27.9

    Human A2M/Alpha2-Macroglobulin ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20min)
    Concentration(pg/ml)0625125025005000100002000040000
    O.D.0.0750.1290.2030.3320.5751.1081.4772.135
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

典型操作程序:

准备试剂和标准品

每孔添加100μl样品和标准品,37℃反应90分钟,不洗。

每孔添加100μl生物素标记抗体,37℃反应60分钟。

0.01M TBS洗涤3次。

每孔添加100μlABC,37℃反应30分钟。

0.01M TBS洗涤5次。

7 TMB37℃避光反应30分钟以内。

加入TMB终止液,读数。

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