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Goat Anti-Mouse IgM Secondary Antibody, HRP Conjugate

HRP-羊抗小鼠IgM;过氧化物酶标记羊抗小鼠IgM

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二抗 - HRP标记二抗

说明书:

BA1075

130元/100μl  

WB

HRP Conjugated

现货

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总价格:

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Product Brief

  • Product Overview

    Product NameGoat Anti-Mouse IgM Secondary Antibody, HRP Conjugate
    SynonymsHRP-conjugated goat anti-mouse IgM
    DescriptionGoat Anti-Mouse IgM Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dot Blot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
    Reagent TypeSecondary antibody, reporter enzyme labeled
    LabelHRP (Horseradish Peroxidase)
    HostGoat
    Target SpeciesMouse
    Antibody ClassIgG
    ClonalityPolyclonal
    ImmunogenWhole molecule mouse IgM
    PurificationImmunoaffinity Chromatography
    SpecificyityMouse IgM specific; No cross-reactivity with mouse IgG
    Form SuppliedLiquid: concentrated buffered stock solution
    Formulation0.25 mg HRP-conjugated secondary antibody 
    0.01 M PBS (PH 7.4) 
    50% glycerol
    Pack Size100μl
    Concentration1 mg/ml
    ApplicationELISA*, WB*, Dot blot 
    Storage4C for 1 year
    PrecautionsFOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

    Assay Information

    Sample TypeSDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
    Assay TypeImmunoassay
    Assay PurposeProtein detection/quantification
    TechniqueImmunodetection of target antibody with HRP reporter enzyme
    Equipment NeededWB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
    Compatibility with ReagentsIncompatible with sodium azide and metals 
    incompatible with high phosphate concentrations

    Main Advantages

    SpecificHigh signal-to-noise ratio
    SensitiveDetects low-abundant targets due to an optimal number of HRP molecules per antibody
    High Signal AmplificationMultiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
    FastGenerates strong signals in a relatively short time span
    QuantifieableAllows quantification of detected signal
    Easy to UseSupplied in a workable liquid format
    FlexibleHRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
    ConvenientHRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

    Background

    Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

    Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.


Instructions

参考效价:
DAB:1:300—1000
ECM:1:2000—4000
ELISA:1:5000—10000

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