产品中心

当前位置:首页>产品中心 >二抗 >荧光FITC标记二抗

Goat Anti-Human IgM Secondary Antibody, FITC Conjugate

FITC-羊抗人IgM,荧光(FITC)标记羊抗人IgM

分类:

二抗 - 荧光FITC标记二抗

说明书:

BA1116

160元/100μl  

Flow Cytometry, IHC, ICC

FITC Conjugate

现货

选择规格:

总价格:

---元*1

选择数量:

- +

Product Brief

  • Product Overview

    Product NameGoat Anti-Human IgM Secondary Antibody, FITC Conjugate
    SynonymsFITC-conjugated Goat Anti-Human IgM; Goat Anti-Human IgM-FITC Secondary Antibody; Fluorescein-labeled Goat Anti-Human IgM Secondary Antibody
    DescriptionGoat Anti-Human IgM Secondary Antibody, FITC Conjugate, for detection, localization and quantification of target proteins in a sample via indirect immunofluorescence in IHC-P, IHC-F, ICC, or FCM.
    Reagent TypeFluorophore-conjugated secondary antibody
    LabelFITC
    HostGoat
    Target SpeciesHuman
    Antibody ClassIgG
    ClonalityPolyclonal
    ImmunogenWhole molecule human IgM
    PurificationImmunoaffinity chromatography
    SpecificityHuman IgM specific;No cross-reactivity with human IgA/IgG
    Form SuppliedLiquid, concentrated buffered stock solution
    Formulation0.5 mg FITC-conjugated secondary antibody 
    0.01 M PBS (PH 7.4) 
    0.01% Thimerosal 
    50% glycerol
    Pack Size100μl
    Concentration1 mg/ml
    ApplicationFlow Cytometry (FCM), Immunohistochemistry (paraffin-embedded (IHC-P) and frozen(IHC-F) sections), Immunocytochemistry (ICC) 
    Storage4C for 1 year
    PrecautionsFOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

    Assay Information

    Sample TypeHuman primary-antibody-probed Single cell suspension, Formalin-fixed paraffin-embedded (FFPE) tissue sections, Thawed frozen samples (IHC-F)
    Assay TypeImmunoanalytical
    Assay PurposeProtein detection/quantification
    TechniqueImmunofluorescence
    Equipment NeededExcitation light source; Filter set and detector: fluorescence microscope (can be combined with confocal microscope), fluorescence plate-reader, flow cytometer, or cell sorter
    Additional Materials RequiredPrimary antibody against target antigen raised in human; Diluent Buffer (PBS or TBS); Application-specific reagents and appliances;

    Main Advantages

    SpecificHigh signal-to-noise ratio
    High Signal AmplificationMultiple secondary antibodies can bind to a single primary antibody; Multiple FITC molecules bind to a single secondary antibody
    FastFewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly
    QuantifieableAllows quantification of detected signal
    Easy to UseSupplied in a workable liquid format
    Multiplex CompatibilityColocalization studies possible, even in close proximity: use primary antibodies from different host species for simultaneous detection by fluorophore-conjugated secondary antibodies; use multiple differently colored fluorophores in the same experiment for target differentiation
    Dynamic rangeGood linearity within detection limits

    Background

    Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

    Immunofluorescence is a technique used for light microscopy with a fluorescence microscope which utilizes fluorescent dyes as reporters. It is being employed in a variety of applications such as cellular imaging and flow cytometry and is commonly used to visualize the distribution of target molecules through a sample, to detect protein location and activation, to identify protein complex formation and conformational changes, and to monitor biological processes in vivo. Fluorescent dyes (also known as fluorochromes, fluorophores, or simply fluors) are molecules that can absorb light of a specific energy and wavelength, thereby undergoing excitation, and then re-emit it at a lower energy and longer wavelength upon returning to the ground state.

    Fluorescent reporters widely used in biological research are of two types: organic compounds with a low molecular weight (0.2-1 kDa) typically containing numerous aromatic groups or plane or cyclic moieties with π bonds (e.g.FITC, TRITC, Alexa Fluor Dyes, DyLight Fluors), and biological fluorophores (e.g.green fluorescent protein (GFP), R-Phycoerythrin). FITC (Fluorescein isothiocyanate) is derivative of fluorescein where a hydrogen atom on the bottom ring of the structure is replaced by isothiocyanate functional group (-N=C=S), making it more reactive to amine and sulfhydryl groups on proteins. The excitation and emission wavelengths of FITC are 495 nm and 525 nm respectively. Like most fluorochromes, it is prone to photobleaching, i.e. losing fluorescing properties due to molecule structure degradation. Loss of activity caused by photobleaching can be controlled by reducing the intensity or time-span of light exposure, by increasing the concentration of the fluorophore, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Analogs of FITC with greater photostability and higher fluorescence intensity tailored in various biological applications are Alexa 488 and DyLight 488.

Instructions

1:用于免疫细胞化学时,中性PBS,TBS稀释。临用前配制。
2:效价:1:32——1:64。

Related products

暂无相关产品