产品中心

当前位置:首页>产品中心 >二抗 >荧光FITC标记二抗

Goat Anti-Mouse IgG(H+L) Secondary Antibody, FITC Conjugate

FITC-羊抗小鼠IgG,荧光(FITC)标记羊抗小鼠IgG

分类:

二抗 - 荧光FITC标记二抗

说明书:

PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 μg/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody (Catalog # BA1101) was used to detect the primary antibody at 30 μg/mL.
  • PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 μg/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody (Catalog # BA1101) was used to detect the primary antibody at 30 μg/mL.

BA1101

140元/100μl  

Flow Cytometry, IHC, ICC

FITC Conjugate

现货

选择规格:

总价格:

---元*1

选择数量:

- +

Product Brief

  • Product Overview

    Product NameGoat Anti-Mouse IgG(H+L) Secondary Antibody, FITC Conjugate
    SynonymsFITC-conjugated Goat Anti-mouse IgG; Goat Anti-Mouse IgG-FITC Secondary Antibody; Fluorescein-labeled Goat Anti-Mouse IgG Secondary Antibody;ee
    DescriptionGoat Anti-Mouse IgG Secondary Antibody, FITC Conjugate, for detection, localization and quantification of target proteins in a sample via indirect immunofluorescence in IHC-P, IHC-F, ICC, or FCM
    Reagent TypeFluorophore-conjugated secondary antibody
    LabelFITC
    HostGoat
    Target SpeciesMouse
    Antibody ClassIgG
    ClonalityPolyclonal
    ImmunogenWhole molecule mouse IgG
    PurificationImmunoaffinity chromatography
    Solid Phase AdsorbtionHuman serum proteins
    SpecificityMouse IgG specific
    Form SuppliedNo cross-reactivity with human/bovine/rabbit IgG
    Formulation0.5 mg FITC-conjugated secondary antibody 
    0.01 M PBS (PH 7.4) 
    0.01% Thimerosal 
    50% glycerol
    Pack Size100μl
    Concentration1 mg/ml
    ApplicationFlow Cytometry (FCM), Immunohistochemistry (paraffin-embedded (IHC-P) and frozen(IHC-F) sections), Immunocytochemistry (ICC) 
    Storage4C for 1 year
    PrecautionsFOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

    Assay Information

    Sample TypeCell suspension, FFPE tissue sections, thawed frozen samples
    Assay TypeImmunoanalytical
    Assay PurposeProtein detection/quantification
    TechniqueImmunodetection of target antibody with HRP reporter enzyme
    Equipment NeededExcitation light source; Filter set and detector: fluorescence microscope (can be combined with confocal microscope), fluorescence plate-reader, flow cytometer, or cell sorter;

    Main Advantages

    SpecificHigh signal-to-noise ratio
    FastFewer number of processing steps - no need for adding a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly
    High Signal AmplificationMultiple secondary antibodies can bind to a single primary antibody; Multiple FITC molecules bind to a single secondary antibody;
    High Image QualityHigh-resolution compatibility with fluorescent microscopy; compatible with multi-planar microscopy
    Precise target localizationSharp, precise signal development
    Multiplex CompatibilityColocalization studies: use primary antibodies from different host species for simultaneous detection by fluorophore-conjugated secondary antibodies; use multiple differently colored fluorophores in the same experiment for target differentiation
    QuantifieableRapid and precise quantitative analysis of fluorescent signal

    Background

    Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

    Immunofluorescence is a technique used for light microscopy with a fluorescence microscope which utilizes fluorescent dyes as reporters. It is being employed in a variety of applications such as cellular imaging and flow cytometry and is commonly used to visualize the distribution of target molecules through a sample, to detect protein location and activation, to identify protein complex formation and conformational changes, to monitor biological processes in vivo. 
    Fluorescent dyes (also known as fluorochromes, fluorophores, or simply fluors) are molecules that can absorb light of a specific energy and wavelength, thereby undergoing excitation, and then re-emit it at a lower energy and longer wavelength upon returning to the ground state.

    Goat Anti-Mouse IgG Secondary Antibody, FITC Conjugate Images

    Click the images to enlarge.

    PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 μg/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody (Catalog # BA1101) was used to detect the primary antibody at 30 μg/mL.

    PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 μg/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody (Catalog # BA1101) was used to detect the primary antibody at 30 μg/mL.

Instructions

1:用于免疫细胞化学时,中性PBS,TBS稀释。临用前配制。
2:效价:1:32——1:64。

Related products

暂无相关产品