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DyLight 594—羊抗小鼠(H+L)

DyLight 594—羊抗小鼠(H+L)

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二抗 - A594标记二抗

说明书:

PCNA(BM0104) 人直肠癌 DyLight 594—羊抗小鼠(H+L)
  • PCNA(BM0104) 人直肠癌 DyLight 594—羊抗小鼠(H+L)

BA1141

180元/0.1ml   

IF,ICC,FCM

DyLight 594 Conjugate

5-7天

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Product Brief

  • Introduction

    Goat Anti-mouse IgG (H+L) secondary antibody, DyLight 594 Conjugate contains affinity-purified antibodies with well-characterized specificity for Mouse IgG (whole molecule) and are useful in the detection of their specified targets through IF,ICC,FCM.

    Overview

    Product NameGoat Anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594 Conjugate
    SKU/Catalog NumberBA1141
    DescriptionThis antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.
    Cite This ProductGoat Anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594 Conjugate (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1141)
    HostGoat
    Validated SpeciesMouse
    ApplicationFlow Cytometry, IF, ICC

    *Our Boster Guarantee covers the use of this product in the above tested applications.

    ImmunogenMouse IgG (whole molecule).
    Pack Size0.1ml

    Properties

    ClonalityPolyclonal
    Concentration1mg/ml
    StorageAt 4°C for one year. Protected from light.

    Background

    Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

    Dilution Ratios/Recommended Concentrations

    At Boster we strive to provide the best Goat Anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594 Conjugate by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.

    Recommended dilution ratios are listed below:
    Flowcytometry, 2-20μg/ml
    Immunocytochemistry, 2-20μg/ml
    Immunofluorescence, 2-20μg/ml

    Goat Anti-Rabbit IgG Secondary Antibody, DyLight?488 Conjugate Images

    Click the images to enlarge.

    PCNA(BM0104) 人直肠癌 DyLight 594—羊抗小鼠(H+L)

    PCNA(BM0104) 人直肠癌 DyLight 594—羊抗小鼠(H+L)


Instructions

荧光素:DyLight 594最大吸收波长593nm,最大发射波长618nm具有高荧光强度,灵敏性出色,对抗光漂白的能力强,在大多数条件下都能获得红色荧光,PH值稳定性好,在很宽的PH值范围内都能获得很好的荧光信号。

试剂内容:

1ml DyLight 594标记羊抗小鼠(H+L),含1mg亲和纯化羊抗小鼠(H+L), 0.01M PBS, 1%BSA, 0.01%Thimerosal。

推荐浓度:1:50-500适用于大多数荧光应用。

使用方法:

DyLight 594标记试剂稀释液:PBS或TBS缓冲液,加入试剂级BSA至1%。

使用上述稀释液,具体的稀释度须自己预先确定。稀释后溶液应于当天使用,未用完的应弃置。

*本产品仅供研究用途,不用于诊断或治疗


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