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Goat Anti-Rabbit IgG(H+L) Secondary Antibody, DyLight 488 Conjugate

DyLight 488-羊抗兔IgG,荧光(DyLight 488)标记羊抗兔IgG

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二抗 - 荧光488/550标记二抗

说明书:

GST3/GST was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-GST3/GST Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL. Lamin B1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Lamin B1 Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.
  • GST3/GST was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-GST3/GST Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.
  • Lamin B1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Lamin B1 Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.

BA1127

180元/100μl  

Flow Cytometry, IHC, ICC

DyLight 488 Conjugate

现货

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Product Brief

  • Introduction

    Goat Anti-rabbit IgG(H+L) secondary antibody, DyLight?488 Conjugate contains affinity-purified antibodies with well-characterized specificity for Rabbit IgG (whole molecule) and are useful in the detection of their specified targets through IHC-P,IHC-F,ICC,FCM.

    Overview

    Product NameGoat Anti-Rabbit IgG(H+L) Secondary Antibody, DyLight 488 Conjugate
    SKU/Catalog NumberBA1127
    DescriptionThis antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.
    Cite This ProductGoat Anti-Rabbit IgG Secondary Antibody, DyLight 488 Conjugate (Boster Biological Technology, Wuhan, CHINA, Catalog # BA1127)
    HostGoat
    Validated SpeciesRabbit
    ApplicationFlow Cytometry, IHC, ICC
    ImmunogenRabbit IgG (whole molecule).
    Pack Size100μl

    Properties

    ClonalityPolyclonal
    Concentration1mg/ml
    StorageAt 4°C for one year. Protected from light.

    Background

    Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

    Goat Anti-Rabbit IgG Secondary Antibody, DyLight?488 Conjugate Images

    Click the images to enlarge.

    GST3/GST was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-GST3/GST Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.

    GST3/GST was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-GST3/GST Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.

    Lamin B1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Lamin B1 Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.

    Lamin B1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Lamin B1 Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight?488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.

Instructions

荧光素:DyLight 488 carboxylic acid最大吸收峰 493nm;最大发射峰 518nm。
试剂内容:1ml DyLight 488—羊抗兔IgG含1mg亲和纯化羊抗兔IgG(特异性抗体纯度99%以上),0.01M PBS,15mg/ml BSA, 0.01%Thimerosal。
使用方法:DyLight 488标记试剂稀释液:0.01 M PBS或TBS缓冲液(PH7.0-7.6),加入试剂级BSA至1%。使用上述稀释液,免疫荧光法按1:100—500稀释。此效价仅供参考,具体的稀释度须自己预先确定。稀释后溶液建议现用现配,未用完的应弃置。
注意事项:请离心后,再使用。

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