|Product Name||Goat Anti-Rabbit IgG（H+L） Secondary Antibody, DyLight®488 Conjugate|
|Description||This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.|
|Cite This Product||Goat Anti-Rabbit IgG Secondary Antibody, DyLight®488 Conjugate (Boster Biological Technology, Wuhan, CHINA, Catalog # BA1127)|
|Application||Flow Cytometry, IHC, ICC|
|Immunogen||Rabbit IgG (whole molecule).|
|Storage||At 4°C for one year. Protected from light.|
Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.
GST3/GST was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-GST3/GST Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight®488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.
Lamin B1 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Lamin B1 Antigen Affinity purified polyclonal antibody at 1 μg/mL. DyLight®488 Conjugated Goat Anti-rabbit IgG secondary antibody (Catalog # BA1127) was used to detect the primary antibody at 10 μg/mL.
荧光素：DyLight 488 carboxylic acid最大吸收峰 493nm；最大发射峰 518nm。
试剂内容：1ml DyLight 488—羊抗兔IgG含1mg亲和纯化羊抗兔IgG（特异性抗体纯度99%以上），0.01M PBS，15mg/ml BSA, 0.01%Thimerosal。
使用方法：DyLight 488标记试剂稀释液：0.01 M PBS或TBS缓冲液(PH7.0-7.6)，加入试剂级BSA至1%。使用上述稀释液，免疫荧光法按1：100—500稀释。此效价仅供参考，具体的稀释度须自己预先确定。稀释后溶液建议现用现配，未用完的应弃置。