DNA Damage (8-OHdG)
|Product Name||DNA Damage (8-OHdG) ELISA Kit|
|Description||Colorimetric detection of DNA Damage (8-OHdG). 96wells/kit, with removable strips.|
|Cite This Product||DNA Damage (8-OHdG) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7114)|
|Validated Species||All Animals|
|Cross Reactivity||8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
|Assay Range||0.94 - 60 ng/mL|
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||Cell lysates, Plasma, Sample matrices, Urine|
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Storage||Store at 4°C and -20°C. (All reagents are stable as supplied at 4°C, except the, which should be stored at -20°C. )|
|8-hydroxy-2-deoxy Guanosine : BSA Coated Plate||1 Plate|
|8-hydroxy-2-deoxy Guanosine Standard||1 vial/ 100uL|
|8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody||1 vial/75uL|
|Sample and Standard Diluent||1 vial/50mL|
|8-hydroxy-2-deoxy Guanosine Antibody Diluent||1 vial/13mL|
|Wash Buffer Concentrate||1 vial/50mL|
|TMB Substrate||1 vial/ 13mL|
|Stop Solution||1 vial/ 13mL|
|Plate Cover||2 covers|
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS.
Typical Standard Curve for the DNA Damage (8-OHdG) ELISA Kit (Enzyme-Linked Immunosorbent Assay)–EK7114 Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.94 – 60 ng/ml.
Chemical Equation of the Oxidation of Guanosine
Diagram of the 8-OHdG Competitive ELISA
Urine Spike Assay
Diagram of the Preparation of the 8-OHdG Standards
Diagram of the Triplicate Sample Plate Format
Preview of the Calculations Worksheet