|Product Name||DNA Damage (8-OHdG) ELISA kit|
|Description||Competitive High Sensitivity ELISA kit for Quantitative Detection of 8-hydroxy-2-deoxy Guanosine. 96wells/kit|
|Cite This Product||DNA Damage (8-OHdG) ELISA kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7008)|
|Cross Reactivity||8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
|Assay Range||0.94 - 60 ng/mL|
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||Cell lysates, Plasma, Sample matrices, Urine|
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Storage||Store the kit at 4°C and -20°C. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|8-hydroxy-2-deoxy Guanosine : BSA Coated Plate||12x8x1 Microwells|
|8-hydroxy-2-deoxy Guanosine Standard||1 vial/ 100uL|
|8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody||1 vial/75uL|
|Sample and Standard Diluent||1 vial/50mL|
|8-hydroxy-2-deoxy Guanosine Antibody Diluent||1 vial/13mL|
|Wash Buffer Concentrate||1 vial/50mL|
|TMB Substrate||1 vial/13mL|
|Stop Solution||1 vial/13mL|
|Plate Cover||2 covers|
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS.