|Product Name||Dibromo-tyrosine ELISA Kit|
|Description||Colorimetric detection of Dibromo-tyrosine. 96wells/kit, with removable strips.|
|Cite This Product||Dibromo-tyrosine ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7118)|
|Validated Species||All Animals|
|Cross Reactivity||There is no detectable cross-reactivity.|
|Pack Size||96wells/kit, with removable strips.|
*Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
|Assay Range||0.078 - 5 ?g/mL|
*This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
|Sample Type||Plasma, Urine|
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it.
**For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
|Storage||Store at 4°C and -20°C. (All reagents are stable as supplied at 4°C, except the, which should be stored at -20°C. )|
|Dibromo-tyrosine: BSA Coated Plate||1 Plate|
|Dibromo-tyrosine Standard||1 vial/75 ?l|
|Dibromo-tyrosine: Biotin Conjugated Monoclonal Antibody||1 vial/75?l|
|Streptavidin Poly HRP Concentrate||1 vial/150 ?l|
|Streptavidin Poly HRP Diluent||1 vial/13 ml|
|Sample and Standard Diluent (Red)||1 vial/ 50mL|
|Dibromo-tyrosine Antibody Diluent (Blue)||1 vial/ 13 ml|
|Wash Buffer Concentrate (10X)||1 vial/ 50 ml|
|TMB Substrate||1 vial/ 13 ml|
|Stop Solution||1 vial/ 13 ml|
Dibromo-tyrosine is produced by the oxidative bromination of tyrosine residues. This reaction occurs via eosinophil peroxidase (EPO), an enzyme released by activated eosinophils. Upon activation of eosinophils, a respiratory burst occurs releasing elevated levels of O2 and H202. In the oxidation of tyrosine, EPO utilizes H202 to catalyze the peroxidation of physiological levels of bromine found within plasma to generate the brominating reagent hypobromous acid (HOBr). Eosinophils play an immunomodulatory role through their recruitment to host sites of parasitic invasion. EPO levels also contribute to diseases such as asthma, cancers and allergic disorders where cellular activation is found to occur at pathological sites (6-10). Brominated products such as 3,5-dibromo-tyrosine serve as biological markers for in vivo eosinophil-mediated tissue damage which allows for understanding the overall roll oxidative stress has on pathways implicated in diseased states within organisms.
Typical Standard Curve for the Dibromo-tyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay)–EK7118 Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.078 – 5 ?g/ml.
Chemical Equation of the Bromination of Tyrosine.
Diagram of the Dibromo-tyrosine Competitive ELISA.
Recovery of dibromo-tyrosine from urine. Urine samples were spiked with dibromo-tyrosine, and diluted as described in the Sample Preparation section (page 10) and analyzed using the Dibromo-Tyrosine ELISA kit. The y-intercept corresponds to the amount of dibromo-tyrosine in unspiked urine.
Diagram of the Preparation of the Dibromo-tyrosine Standards.
Diagram of the Triplicate Sample Plate Format.
Graph of the Dibromo-tyrosine Spike Recovery in Human Plasma.
Graph of the sample comparison between diabetic smoker plasma and non-diabetic/non-smoker plasma.