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Rabbit Anti-Human IgG(H+L) Secondary Antibody, Biotin Conjugate

Biotin-兔抗人IgG;生物素标记兔抗人IgG

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二抗 - Biotin标记二抗

说明书:

BA1020

160元/200μl  300元/500μl  

ELISA, IHC-P, IHC-F, ICC, WB

Biotin Conjugated

现货

总价格:

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选择数量:

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Product Brief

  • Product Overview

    Product NameRabbit Anti-Human IgG(H+L) Secondary Antibody, Biotin Conjugate
    SynonymsBiotin-conjugated Rabbit Anti-Human IgG; Rabbit Anti-Human IgG Biotinylated Antibody; Biotinylated Rabbit Anti-Human IgG Secondary Antibody; Rabbit Anti-Human IgG Secondary Antibody, Biotin-labeled
    DescriptionRabbit Anti-Human IgG Secondary Antibody, Biotin Conjugate, for indirect sensitive immunodetection and/or quantification of low-abundance target proteins through ELISA, IHC-P, IHC-F, ICC, or WB by using reporter-labeled biotin-binding signal amplification systems.
    Reagent TypeBiotin conjugated secondary antibody
    ConjugateBiotin
    HostRabbit
    Target SpeciesHuman
    Antibody ClassIgG
    ClonalityPolyclonal
    ImmunogenWhole molecule human IgG
    PurificationImmunoaffinity chromatography
    SpecificityHuman IgG specific; no cross-reactivity with rat/mouse/goat/rabbit IgG
    Form SuppliedLiquid: concentrated buffered stock solution
    Formulation0.5 mg biotin-conjugated secondary antibody 
    0.01 M PBS (PH 7.4) 
    0.01% Thimerosal 
    50% glycerol
    Pack Size200μl/500μl
    Concentration1 mg/ml
    ApplicationELISA, IHC-P, IHC-F, ICC, WB
    Storage4C for 1 year
    PrecautionsFOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

    Assay Information

    Sample TypeHuman primary-antibody-probed: SDS-PAGE separated-, membrane-immobilized-proteins from cell/tissue lysates, formalin-fixed paraffin-embedded (FFPE) tissue sections on slides
    Assay TypeImmunoanalytical
    TechniqueIndirect immunodetection of target protein via reporter-labeled biotin-binding detection systems
    Assay PurposeProtein detection/quantification
    Equipment NeededWB/Dot blot/ELISA/IHC instrumentation; Reporter signal detectors: X-ray film cassette; a charge-coupled device (CCD) imager; Spectrophotometer; fluorescent or electron microscope

    Main Advantages

    SpecificHigh signal-to-noise ratio
    High Signal AmplificationMultiple secondary antibodies can bind to a single primary antibody; Multiple reporter molecules localize to a single biotin via avidin/streptavidin bridges
    FastFewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly
    QuantifieableAllows quantification of detected signal
    Easy to UseSupplied in a workable liquid format
    FlexibleBiotin-(Strept)Avidin system can be coupled with various types of reporters (enzymes, fluorochromes, fluorophores, chromophores, etc.); One type of labeled secondary antibody can be used to recognize different types of primary antibodies of the target organism specific to a particular antigen
    CompatibleBiotin does not interfere with catalysis or antibody binding

    Background

    Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

    Biotinylated antibodies are widely used in systems where signal amplification is desired. Often 15-20 biotin moieties are coupled to a single IgG secondary antibody. Biotin binds avidin, streptavidin, or neutravidin with a high degree of affinity and specificity. In immunoassays avidin/streptavidin-biotin binding is used as a bridge between antibodies and reporters like enzymes (HRP, AP), fluorophores, chromophores, etc. Both avidin and streptavidin are tetrameric proteins capable of binding 4 biotin groups to each molecule of avidin or streptavidin, thus amplifying the signal intensity and detection sensitivity by increasing the concentration of reporters at the antigenic site. Two main biotin-binding detection systems have been widely utilized: Avidin-Biotin Complex (ABC) and Labeled Streptavidin Biotin (LSAB) methods. In the ABC method free avidin (or streptavidin) is used as a bridge/link between the biotinylated antibody and а biotinylated reporter molecule, resulting in three reporter molecules coupled to the biotinylated antibody. The LSAB method employs a reporter-labeled streptavidin (avidin or neutravidin can alternatively be used) to detect the bound biotinylated-secondary antibody on the tissue section, blotting membrane or ELISA plate, improving the sensitivity of detection by 8-fold. The LSAB method is used when the avidin-biotin-enzyme complex in the ABC method becomes too large to penetrate the tissue.

Instructions

用于免疫组化,Western Blotting等,必须配合各种酶,萤光标记的亲和素(链酶亲和素)使用。
参考效价:
免疫组化 1:100—200
免疫印迹(DAB)1:200—400
免疫印迹(ECM)1:3000—10000
ELISA 1:5000—20000


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