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Biotin Conjugated Protein A

Biotin-Protein A;生物素标记Protein A

分类:

二抗 - Biotin标记二抗

说明书:

BA1025

180元/200μl  340元/500μl  

ELISA, IHC, ICC, WB

Biotin Conjugated

现货

总价格:

---元*1

选择数量:

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Product Brief

  • Overview

    Product NameBiotin Conjugated Protein A
    SKU/Catalog NumberBA1025
    DescriptionProtein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.
    Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications.
    Cite This ProductBiotin Conjugated Protein A (Boster Biological Technology, Wuhan, CHINA, Catalog # BA1025)
    ApplicationELISA, IHC, ICC, WB
    ConjugateBiotin
    Pack Size200μl/500μl

    Properties

    Concentration1mg/ml
    StorageAt 4°C for one year.


Instructions

用于免疫组化,Western Blotting等,必须配合各种酶,萤光标记的亲和素(链酶亲和素)使用。
参考效价:
免疫组化 1:100—200
免疫印迹(DAB)1:200—400
免疫印迹(ECM)1:3000—10000
ELISA 1:5000—20000


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