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Anti-GAPDH Mouse Monoclonal Antibody

aging-associated gene 9 protein|G3P|G3PD|GAPD|GAPDH|glyceraldehyde 3-phosphate dehydrogenase|Glyceraldehyde-3-phosphate dehydrogenase|MGC88685

分类:

一抗 - 单克隆抗体

说明书:

Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody. Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.
  • Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.

BM3876

980元/200μl  

human,mouse,rat,monkey,chicken,zebrafish,fish

WB,IHC,IC/IF,IP,FC

Monoclonal

5-7天

选择规格:

总价格:

---元*1

选择数量:

- +

Product Brief

    • 产品概况

      产品名称Anti-GAPDH Mouse Monoclonal Antibody
      文献引用格式Anti-GAPDH Mouse Monoclonal Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM3876)
      验证种属Human,Mouse,Rat,Monkey,Chicken,Zebrafish,Fish
      应用范围WB,IHC,ICC/IF,IP,FC
      抗体亚型Mouse IgG
      免疫原A synthesized peptide derived from human GAPDH Mouse Monoclonal
      分子量36kDa
      抗体规格200μl/980元

      产品详情

      克隆Monoclonal
      产品形态Liquid
      内容Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer            
      *可以提供无载体抗体。
      储存条件Store at -20℃ for one year, at 4℃ for six months. We suggest the antibody be aliquotted into small vials and stored frozen at -20℃ upon receiving. Avoid repeated freezing and thawing.
      纯化状态Affinity-chromatography

      指标相关信息 (来源: Uniprot.org)

      小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

      Uniprot IDP04406
      指标别名aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685;

      指标背景简介

      Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

      产品应用细节

      为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

      WB 1:100-400;IHC 1:10-50;ICC/IF 1:10-50;IP 1:20;FC 1:20

      *最优稀释度需要用户自己调试,此处数据仅供参考。

      **博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

      产品图片

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      Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody.

      Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody.

      Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.

      Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.

    Instructions

    抗体的保存建议:抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!

    1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)

    2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。

    ◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。

    ◇分装的量以一次实验用完为好,但建议最少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)

    ◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。

    ◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!

    ◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。

    3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。

    4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。

    5.特殊抗体的保存:

    ◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。

    ◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

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