产品中心

当前位置:首页>产品中心 >一抗 >单克隆抗体

Anti-beta Actin Mouse Monoclonal Antibody

ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;

分类:

一抗 - 单克隆抗体

说明书:

Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.
  • Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

BM5422

980元/200μl  

Human,Mouse,Monkey,Goat,Rat,Hamster,Saccharomycescerevisiae,Arabidopsis,Pichiapastoris,Rabbit,Zebrafish,Drosophila,LeafofRice,FruitofCucumber,ChlamydomonasReinhardtii

WB

Monoclonal

5-7天

选择规格:

总价格:

---元*1

选择数量:

- +

Product Brief

  • 产品概况

    产品名称 Anti-beta Actin Mouse Monoclonal Antibody
    产品描述 Anti-beta Actin Mouse Monoclonal Antibody tested for WB in Human , Mouse , Monkey , Goat , Rat , Hamster , Saccharomyces cerevisiae , Arabidopsis , Pichia pastoris , Rabbit , Zebrafish , Drosophila , Leaf of Rice , Fruit of Cucumber , Chlamydomonas Reinhardtii
    文献引用格式 Anti-beta Actin Mouse Monoclonal Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM5422)
    验证种属 Human , Mouse , Monkey , Goat , Rat , Hamster , Saccharomyces cerevisiae , Arabidopsis , Pichia pastoris , Rabbit , Zebrafish , Drosophila , Leaf of Rice , Fruit of Cucumber , Chlamydomonas Reinhardtii
    应用范围 WB
    抗体来源 Mouse
    抗体亚型 Mouse IgG
    免疫原 A synthesized peptide derived from human beta Actin
    分子量 42kDa
    抗体规格 200ul/980元

    产品详情

    克隆 Monoclonal
    产品形态 Liquid
    内容 Purified antibody in PBS with 0.05% sodium azide and 0.205% protein stabilizer
    *可以提供无载体抗体。
    储存条件 At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -220℃ for a longer time. Avoid repeated freezing and thawing.
    纯化状态 Affinity-chromatography

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    Uniprot ID P60709
    指标别名 ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;

    指标背景简介

    beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

    产品图片

    点击图片放大
    Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

    Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

Instructions

抗体的保存建议:抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!
1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)
2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。
◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。
◇分装的量以一次实验用完为好,但建议最少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)
◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。
◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!
◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。
3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。
4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。
5.特殊抗体的保存:
◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。
◇偶联抗体:所有偶联抗体需要203℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

Related products

暂无相关产品