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Anti-YWHAZ Antibody(monoclonal, 6G5)

14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ

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一抗 - 单克隆抗体

说明书:

Figure 1. Western blot analysis of anti-YWHAZ antibody (M01141). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Hela whole cell lysates,<br>Lane 2: A549 whole cell lysates,<br>Lane 3: COS-7 whole cell lysates,<br>Lane 4: Raji whole cell lysates,<br>Lane 5: Caco-2 whole cell lysates,<br>Lane 6: Jurkat whole cell lysates,<br>Lane 7: mouse brain lysates,<br>Lane 8: rat brain lysates,<br>Use rabbit anti-YWHAZ 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAZ at approximately 28KD. The expected band size for YWHAZ is at 28KD. Figure 2. Flow cytometry analysis of PC-3 cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line). Figure 3. Flow cytometry analysis of SiHa cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
  • Figure 1. Western blot analysis of anti-YWHAZ antibody (M01141). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Hela whole cell lysates,<br>Lane 2: A549 whole cell lysates,<br>Lane 3: COS-7 whole cell lysates,<br>Lane 4: Raji whole cell lysates,<br>Lane 5: Caco-2 whole cell lysates,<br>Lane 6: Jurkat whole cell lysates,<br>Lane 7: mouse brain lysates,<br>Lane 8: rat brain lysates,<br>Use rabbit anti-YWHAZ 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAZ at approximately 28KD. The expected band size for YWHAZ is at 28KD.
  • Figure 2. Flow cytometry analysis of PC-3 cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
  • Figure 3. Flow cytometry analysis of SiHa cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

M01141

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,FCM

Monoclonal(Clone: 6G5)

现货

总价格:

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Product Brief

  • 产品概况

    货号 M01141
    产品名称 Anti-YWHAZ Antibody(monoclonal, 6G5)
    基因名 YWHAZ
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 6G5)
    抗体亚型 Mouse IgG2b
    分子量 28KD
    免疫原 A synthetic peptide corresponding to a sequence of human 14-3-3 zeta/delta (LLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQ).
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 14-3-3 protein zeta/delta (14-3-3ζ) is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
    研究类别 1. "Entrez Gene: YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide".2. Nishimura Y, Komatsu S, Ichikawa D, Nagata H, Hirajima S, Takeshita H, Kawaguchi T, Arita T, Konishi H, Kashimoto K, Shiozaki A, Fujiwara H, Okamoto K, Tsuda H, Otsuji E (Apr 2013). "Overexpression of YWHAZ relates to tumor cell proliferation and malignant outcome of gastric carcinoma". British Journal of Cancer. 108 (6): 1324–31.
    Uniprot ID YWHAZ: P63104
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti-YWHAZ antibody (M01141). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Hela whole cell lysates,<br>Lane 2: A549 whole cell lysates,<br>Lane 3: COS-7 whole cell lysates,<br>Lane 4: Raji whole cell lysates,<br>Lane 5: Caco-2 whole cell lysates,<br>Lane 6: Jurkat whole cell lysates,<br>Lane 7: mouse brain lysates,<br>Lane 8: rat brain lysates,<br>Use rabbit anti-YWHAZ 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAZ at approximately 28KD. The expected band size for YWHAZ is at 28KD.

    Figure 1. Western blot analysis of anti-YWHAZ antibody (M01141). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Hela whole cell lysates,
    Lane 2: A549 whole cell lysates,
    Lane 3: COS-7 whole cell lysates,
    Lane 4: Raji whole cell lysates,
    Lane 5: Caco-2 whole cell lysates,
    Lane 6: Jurkat whole cell lysates,
    Lane 7: mouse brain lysates,
    Lane 8: rat brain lysates,
    Use rabbit anti-YWHAZ 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAZ at approximately 28KD. The expected band size for YWHAZ is at 28KD.

    Figure 2. Flow cytometry analysis of PC-3 cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

    Figure 2. Flow cytometry analysis of PC-3 cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

    Figure 3. Flow cytometry analysis of SiHa cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

    Figure 3. Flow cytometry analysis of SiHa cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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