14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ
|产品名称||Anti-YWHAZ Antibody(monoclonal, 6G5)|
|免疫原||A synthetic peptide corresponding to a sequence of human 14-3-3 zeta/delta (LLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQ).|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||14-3-3 protein zeta/delta (14-3-3ζ) is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.|
|研究类别||1. "Entrez Gene: YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide".2. Nishimura Y, Komatsu S, Ichikawa D, Nagata H, Hirajima S, Takeshita H, Kawaguchi T, Arita T, Konishi H, Kashimoto K, Shiozaki A, Fujiwara H, Okamoto K, Tsuda H, Otsuji E (Apr 2013). "Overexpression of YWHAZ relates to tumor cell proliferation and malignant outcome of gastric carcinoma". British Journal of Cancer. 108 (6): 1324–31.|
|Uniprot ID||YWHAZ: P63104|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Flow Cytometry:||1-3μg/1x106 cells|
Figure 1. Western blot analysis of anti-YWHAZ antibody (M01141). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Hela whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: COS-7 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: Caco-2 whole cell lysates,
Lane 6: Jurkat whole cell lysates,
Lane 7: mouse brain lysates,
Lane 8: rat brain lysates,
Use rabbit anti-YWHAZ 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAZ at approximately 28KD. The expected band size for YWHAZ is at 28KD.
Figure 2. Flow cytometry analysis of PC-3 cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 3. Flow cytometry analysis of SiHa cell HELA(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).