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Anti-5-Lipoxygenase(Ser523) Antibody,Phospho-Specific

Arachidonate 5-lipoxygenase|5-LO|5-lipoxygenase|1.13.11.34|ALOX5|LOG5

分类:

一抗 - 多克隆抗体

说明书:

Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
  • Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).

P00847

3600元/100μl  

human,rat

IHC,WB

Polyclonal

3-4周

选择规格:

总价格:

---元*1

选择数量:

- +

Product Brief

    • 产品概况

      产品名称5-Lipoxygenase(Ser523) Antibody, Phospho-Specific
      产品描述Phospho-specific Rabbit Anti-5-Lipoxygenase (Ser523) Antibody validated for WB in human, rat.
      文献引用格式5-Lipoxygenase(Ser523) Antibody, Phospho-Specific (Boster Biological Technology, Wuhan, China. Catalog #P00847)
      抗体来源Rabbit
      免疫原The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-lipoxygenase (5-LO).
      抗体规格100μl

      产品详情

      克隆Polyclonal
      产品形态Liquid
      内容100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.            
      *可以提供无载体抗体。
      储存条件At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time.Avoid repeated freezing and thawing.

      指标相关信息 (来源: Uniprot.org)

      小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

      基因名ALOX5
      基因全名Arachidonate 5-lipoxygenase
      蛋白功能Catalyzes the first step in leukotriene biosynthesis,and thereby plays a role in inflammatory processes..
      Uniprot IDALOX5: P09917

      产品图片

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      Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).

      Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).

    Instructions

    抗体的保存建议:抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!

    1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)

    2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。

    ◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。

    ◇分装的量以一次实验用完为好,但建议最少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)

    ◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。

    ◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!

    ◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。

    3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。

    4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。

    5.特殊抗体的保存:

    ◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。

    ◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

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