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4E-BP1 Antibody

Eukaryotic translation initiation factor 4E-binding protein 1|BP-1|4EBP1|4E-BP1|PHAS-I|EIF4EBP1

分类:

一抗 - 单克隆抗体

说明书:

4E-BP1(BM1637)(MW:28KD)A431细胞裂解,免疫印迹分析。 Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.
  • 4E-BP1(BM1637)(MW:28KD)A431细胞裂解,免疫印迹分析。
  • Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.

BM1637

980元/0.2ml

human

WB,IHC-P,ELISA

Monoclonal

5-7天

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总价格:

---元*1

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Product Brief

  • 产品概况

    产品名称 4E-BP1 Antibody
    产品描述 Anti-4E-BP1 Antibody validated for IHC, WB, ELISA in Human.
    文献引用格式 4E-BP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM1637)
    应用范围 IHC, WB, ELISA
    抗体来源 Mouse
    抗体亚型 IgG1
    免疫原 Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
    抗体规格 0.2ml/980元

    产品详情

    克隆 Monoclonal
    克隆号 单克隆抗体:IgG1;克隆号:4B6G10
    产品形态 Liquid
    内容 Ascitic fluid containing 0.03% sodium azide.
    *可以提供无载体抗体。
    储存条件 连续使用时4°C存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20°C存储,并避免反复冻融,保质期一年。

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 EIF4EBP1
    基因全名 Eukaryotic translation initiation factor 4E-binding protein 1
    Tissue Specificity Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. .
    Sequence Similarities Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I;EIF4EBP1;
    Uniprot ID EIF4EBP1: Q13541

    指标背景简介

    4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

    产品图片

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    4E-BP1(BM1637)(MW:28KD)A431细胞裂解,免疫印迹分析。

    4E-BP1(BM1637)(MW:28KD)A431细胞裂解,免疫印迹分析。

    Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.

Instructions

抗体的保存建议: 抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!
1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)
2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。
◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。
◇分装的量以一次实验用完为好,但建议最少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)
◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。
◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!
◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。
3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。
4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。
5.特殊抗体的保存:
◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。
◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

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