14-3-3 protein sigma|Epithelial cell marker protein 1|Stratifin|SFN|HME1
|产品名称||14-3-3 sigma antibody|
|产品描述||Rabbit IgG polyclonal antibody for 14-3-3 sigma detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.|
|文献引用格式||14-3-3 sigma antibody (Boster Biological Technology, Wuhan, China. Catalog #A01127)|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide 可以联系我们购买。
|免疫原||E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).|
|交叉反应||No cross reactivity with other proteins.|
|内容||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
|储存条件||At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.|
|蛋白功能||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.|
|Tissue Specificity||Present mainly in tissues enriched in stratified squamous keratinizing epithelium.|
|Uniprot ID||SFN: P31947|
Figure 1. Western blot analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human placenta tissue lysate, Lane 3: human MCF-7 cell lysate, Lane 4: human 22RV1 cell lysate, Lane 5: rat tongue tissue lysate, Lane 6: rat lung tissue lysate, Lane 7: rat PC-12 cell lysate, Lane 8: mouse lung tissue lysate, Lane 9: mouse HEPA1-6 cell lysate, Lane 10: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma at approximately 28KD. The expected band size for 14-3-3 sigma is at 28KD.
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