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14-3-3 sigma antibody

14-3-3 protein sigma|Epithelial cell marker protein 1|Stratifin|SFN|HME1

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一抗 - 多克隆抗体

说明书:

Figure 1. Western blot analysis of 14-3-3 sigma  using anti-14-3-3 sigma  antibody (A01127). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
Lane 1: human Hela cell lysate,
Lane 2: human placenta tissue lysate,
Lane 3: human MCF-7 cell lysate,
Lane 4: human 22RV1 cell lysate,
Lane 5: rat tongue tissue lysate,
Lane 6: rat lung tissue lysate,
Lane 7: rat PC-12 cell lysate,
Lane 8: mouse lung tissue lysate,
Lane 9: mouse HEPA1-6 cell lysate,
Lane 10: mouse NIH3T3 cell lysate. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma  antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma  at approximately 28KD. The expected band size for 14-3-3 sigma  is at 28KD.
  • Figure 1. Western blot analysis of 14-3-3 sigma  using anti-14-3-3 sigma  antibody (A01127). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
Lane 1: human Hela cell lysate,
Lane 2: human placenta tissue lysate,
Lane 3: human MCF-7 cell lysate,
Lane 4: human 22RV1 cell lysate,
Lane 5: rat tongue tissue lysate,
Lane 6: rat lung tissue lysate,
Lane 7: rat PC-12 cell lysate,
Lane 8: mouse lung tissue lysate,
Lane 9: mouse HEPA1-6 cell lysate,
Lane 10: mouse NIH3T3 cell lysate. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma  antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma  at approximately 28KD. The expected band size for 14-3-3 sigma  is at 28KD.

A01127

1680元/100ug

human,mouse,rat

WB,ELISA

Polyclonal

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 14-3-3 sigma antibody
    产品描述 Rabbit IgG polyclonal antibody for 14-3-3 sigma detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.
    文献引用格式 14-3-3 sigma antibody (Boster Biological Technology, Wuhan, China. Catalog #A01127)
    应用范围 ELISA, WB
    抗体来源 Rabbit
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
    *Blocking peptide 可以联系我们购买。
    免疫原 E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).
    交叉反应 No cross reactivity with other proteins.
    抗体规格 100μg/vial

    产品详情

    克隆 Polyclonal
    产品形态 Lyophilized
    内容 Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    *可以提供无载体抗体。
    储存条件 At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 SFN
    基因全名 stratifin
    蛋白功能 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
    Tissue Specificity Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
    Uniprot ID SFN: P31947

    指标背景简介

    Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

    产品图片

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    Figure 1. Western blot analysis of 14-3-3 sigma  using anti-14-3-3 sigma  antibody (A01127). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
Lane 1: human Hela cell lysate,
Lane 2: human placenta tissue lysate,
Lane 3: human MCF-7 cell lysate,
Lane 4: human 22RV1 cell lysate,
Lane 5: rat tongue tissue lysate,
Lane 6: rat lung tissue lysate,
Lane 7: rat PC-12 cell lysate,
Lane 8: mouse lung tissue lysate,
Lane 9: mouse HEPA1-6 cell lysate,
Lane 10: mouse NIH3T3 cell lysate. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma  antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma  at approximately 28KD. The expected band size for 14-3-3 sigma  is at 28KD.

    Figure 1. Western blot analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human placenta tissue lysate, Lane 3: human MCF-7 cell lysate, Lane 4: human 22RV1 cell lysate, Lane 5: rat tongue tissue lysate, Lane 6: rat lung tissue lysate, Lane 7: rat PC-12 cell lysate, Lane 8: mouse lung tissue lysate, Lane 9: mouse HEPA1-6 cell lysate, Lane 10: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma antigen affinity purified polyclonal antibody (Catalog # A01127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 sigma at approximately 28KD. The expected band size for 14-3-3 sigma is at 28KD.

Instructions

抗体的保存建议: 抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!
1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)
2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。
◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。
◇分装的量以一次实验用完为好,但建议最少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)
◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。
◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!
◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。
3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。
4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。
5.特殊抗体的保存:
◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。
◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

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