Arachidonate 12-lipoxygenase|12S-type|12S-LOX|12S-lipoxygenase|18.104.22.168|Lipoxin synthase 12-LO|3.3.2.-|Platelet-type lipoxygenase 12|ALOX12|12LO|LOG12|
|产品名称||12 Lipoxygenase Antibody|
|产品描述||Rabbit IgG polyclonal antibody for Arachidonate 12-lipoxygenase, 12S-type(ALOX12) detection. Tested with WB in Human,Mouse,Rat.|
|文献引用格式||12 Lipoxygenase Antibody (Boster Biological Technology, Wuhan, China. Catalog #A02275-1)|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide 可以联系我们购买。￥300/1ml
|免疫原||A synthetic peptide corresponding to a sequence at the N-terminus of human 12 Lipoxygenase (186-231aa ALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQ), different from the related mouse sequence by six amino acids, and from the related rat sequence by seven|
|交叉反应||No cross reactivity with other proteins.|
|内容||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
|浓度||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|储存条件||At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time.Avoid repeated freezing and thawing.|
|基因全名||Arachidonate 12-lipoxygenase, 12S-type|
|蛋白功能||Non-heme iron-containing dioxygenase that catalyzes thestereo-specific peroxidation of free and esterifiedpolyunsaturated fatty acids generating a spectrum of bioactivelipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can alsometabolize linoleic acid. Has a dual activity since it alsoconverts leukotriene A4/LTA4 into both the bioactive lipoxinA4/LXA4 and lipoxin B4/LXB4. Through the production of specificbioactive lipids like (12S)-HPETE it regulates differentbiological processes including platelet activation. It alsoprobably positively regulates angiogenesis through regulation ofthe expression of the vascular endothelial growth factor. Plays arole in apoptotic process, promoting the survival of vascularsmooth muscle cells for instance. May also play a role in thecontrol of cell migration and proliferation..|
|Tissue Specificity||Expressed in vascular smooth muscle cells..|
|Uniprot ID||ALOX12: P18054|
Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates, lane 3: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75KD.
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