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12 Lipoxygenase Antibody

Arachidonate 12-lipoxygenase|12S-type|12S-LOX|12S-lipoxygenase|1.13.11.31|Lipoxin synthase 12-LO|3.3.2.-|Platelet-type lipoxygenase 12|ALOX12|12LO|LOG12|

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一抗 - 多克隆抗体

说明书:

Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
lane 1: rat spleen tissue lysates,
lane 2: mouse spleen tissue lysates,
lane 3: COLO320 whole cell lysates. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75KD.
  • Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
lane 1: rat spleen tissue lysates,
lane 2: mouse spleen tissue lysates,
lane 3: COLO320 whole cell lysates. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75KD.

A02275-1

1680元/100ug

human,mouse,rat

WB

Polyclonal

现货

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总价格:

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Product Brief

  • 产品概况

    产品名称 12 Lipoxygenase Antibody
    产品描述 Rabbit IgG polyclonal antibody for Arachidonate 12-lipoxygenase, 12S-type(ALOX12) detection. Tested with WB in Human,Mouse,Rat.
    文献引用格式 12 Lipoxygenase Antibody (Boster Biological Technology, Wuhan, China. Catalog #A02275-1)
    应用范围 WB
    抗体来源 Rabbit
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
    *Blocking peptide 可以联系我们购买。¥300/1ml
    免疫原 A synthetic peptide corresponding to a sequence at the N-terminus of human 12 Lipoxygenase (186-231aa ALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQ), different from the related mouse sequence by six amino acids, and from the related rat sequence by seven
    交叉反应 No cross reactivity with other proteins.
    抗体规格 100ug/vial

    产品详情

    克隆 Polyclonal
    产品形态 Lyophilized
    内容 Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    *可以提供无载体抗体。
    浓度 Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
    储存条件 At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time.Avoid repeated freezing and thawing.

    指标相关信息 (来源: Uniprot.org)

    小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
    基因名 ALOX12
    基因全名 Arachidonate 12-lipoxygenase, 12S-type
    蛋白功能 Non-heme iron-containing dioxygenase that catalyzes thestereo-specific peroxidation of free and esterifiedpolyunsaturated fatty acids generating a spectrum of bioactivelipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can alsometabolize linoleic acid. Has a dual activity since it alsoconverts leukotriene A4/LTA4 into both the bioactive lipoxinA4/LXA4 and lipoxin B4/LXB4. Through the production of specificbioactive lipids like (12S)-HPETE it regulates differentbiological processes including platelet activation. It alsoprobably positively regulates angiogenesis through regulation ofthe expression of the vascular endothelial growth factor. Plays arole in apoptotic process, promoting the survival of vascularsmooth muscle cells for instance. May also play a role in thecontrol of cell migration and proliferation..
    Tissue Specificity Expressed in vascular smooth muscle cells..
    Uniprot ID ALOX12: P18054

    指标背景简介

    ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNA Arachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.

    产品图片

    点击图片放大
    Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. 
lane 1: rat spleen tissue lysates,
lane 2: mouse spleen tissue lysates,
lane 3: COLO320 whole cell lysates. 
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75KD.

    Figure 1. Western blot analysis of 12 Lipoxygenase using anti-12 Lipoxygenase antibody (A02275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysates, lane 2: mouse spleen tissue lysates, lane 3: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-12 Lipoxygenase antigen affinity purified polyclonal antibody (Catalog # A02275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 12 Lipoxygenase at approximately 80KD. The expected band size for 12 Lipoxygenase is at 75KD.

Instructions

抗体的保存建议: 抗体保存得当与否直接决定了抗体的活性和使用效果。如果抗体保存得当,大部分抗体活性都可以维持数月甚至数年。请按照说明书或者抗体标签上推荐的保存条件正确保存抗体!无论是保存还是运输,绝对避免反复冻融抗体!
1.拿到抗体后请务必在1000-3000转离心1-2分钟后再打开管盖进行分装和保存!(由于运输过程中反复颠簸,管内微量抗体容易聚集在管盖处,一旦直接打开容易流失抗体。请放心博士德抗体出厂时保证已装入足量。)
2.对于博士德大部分抗体,比较合适的保存方式是分装后保存在-20℃冰箱。
◇分装可以最大程度降低反复冻融对抗体活性的损害,同时也降低了多次从同一管中吸取抗体造成污染的可能性。
◇分装的量以一次实验用完为好,但建议最少不要少于10μL每份。分装体积越小,抗体浓度可能会受到蒸发以及管壁吸附的影响,同时分装次数越多移液吸头吸附的抗体也越多,可能会误认为抗体没有足量装入。(最好用无菌的进口0.2ML离心管来分装抗体,国产的小管一般没有经过很好的硅化处理,容易吸附抗体蛋白,影响抗体活性。)
◇分装时请将无菌的微量移液器吸头先插入管底部反复吹打几次后再吸出抗体,以便抗体有效成分与抗体保护剂充分混匀。
◇复融后的分装抗体如果一次用不完,请将剩余母液保存在4℃冰箱,避免再冻起来!
◇绝对避免将抗体保存在自动除霜冰箱的冷藏室中。尽量将抗体保存在手动除霜冰箱里面,而不是在冰箱门上。
3.大部分抗体收到后4℃短暂保存1-2周对抗体活性是基本没有影响的。如果抗体很快(1-2周内)就能使用完,推荐在4℃保存,避免反复冻融对抗体活性的损害。如果要长期保存则分装后-20℃保存。
4.即用型抗体请务必保存在4℃冰箱,一定不能冷冻保存。
5.特殊抗体的保存:
◇酶联抗体:一般保存在4℃,尽量避免冷冻起来。否则可能会导致酶活力的下降或者丧失。
◇偶联抗体:所有偶联抗体需要4℃避光保存。尤其是荧光标记抗体,对光极为敏感,因此所有实验阶段也是要避光操作的。

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